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Tsinghua Science and Technology

Keywords

mass spectrometry, Fourier transform, noise filtering, Markov Chain Monte Carlo (MCMC)

Abstract

In proteomics, many methods for the identification of proteins have been developed. However, because of limited known genome sequences, noisy data, incomplete ion sequences, and the accuracy of protein identification, it is challenging to identify peptides using tandem mass spectral data. Noise filtering and removing thus play a key role in accurate peptide identification from tandem mass spectra. In this paper, we employ a Bayesian model to identify proteins based on the prior information of bond cleavages. A Markov Chain Monte Carlo (MCMC) algorithm is used to simulate candidate peptides from the posterior distribution and to estimate the parameters for the Bayesian model. Our simulation and computational experimental results show that the model can identify peptide with a higher accuracy.

Publisher

Tsinghua University Press

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