Facile synthesis of wormlike quantum dots-encapsulated nanoparticles and their controlled surface functionalization for effective bioapplications
quantum dots, morphology, poly(glycidyl methacrylate)(PGMA), gene delivery, imaging
Semiconductor quantum dots (QDs) are considered as ideal fluorescent probesowing to their intrinsic optical properties. It has been demonstrated that the sizeand shape of nanoparticles significantly influence their behaviors in biologicalsystems. In particular, one-dimensional (1D) nanoparticles with larger aspectratios are desirable for cellular uptake. Here, we explore a facile and greenmethod to prepare novel 1D wormlike QDs@SiO2 nanoparticles with controlledaspect ratios, wherein multiple QDs are arranged in the centerline of the nanoparticles.Then, an excellent cationic gene carrier, ethanolamine-functionalizedpoly(glycidyl methacrylate) (denoted by BUCT-PGEA), was in-situ produced viaatom transfer radical polymerization on the surface of the QDs@SiO2 nanoparticlesto achieve stable surfaces (QDs@SiO2-PGEA) for effective bioapplications. Wefound that the wormlike QDs@SiO2-PGEA nanoparticles demonstrated muchhigher gene transfection performance than ordinary spherical counterparts. Inaddition, the wormlike nanoparticles with larger aspect ratio performed betterthan those with smaller ratio. Furthermore, the gene delivery processes includingcell entry and plasmid DNA (pDNA) escape and transport were also trackedin real time by the QDs@SiO2-PGEA/pDNA complexes. This work realized theintegration of efficient gene delivery and real-time imaging within one controlled1D nanostructure. These constructs will likely provide useful informationregarding the interaction of nanoparticles with biological systems.
Tsinghua University Press
Yajuan Yang,Yu Qi,Min Zhu,Nana Zhao,Fujian Xu, Facile synthesis of wormlike quantum dots-encapsulated nanoparticles and their controlled surface functionalization for effective bioapplications. NanoRes.2016, 9(9): 2531–2543