Prolonging the plasma circulation of proteins by nano-encapsulation with phosphorylcholine-based polymer
phosphorylcholine-basedpolymer, nano-encapsulation, function protein delivery, protein therapy, long-circulation
Short in vivo circulation is a major hindrance to the widespread adoption of protein therapeutics. Protein nanocapsules generated by encapsulating proteins with a thin layer of phosphorylcholine-based polymer via a two-step encapsulation process exhibited significantly prolonged plasma half-life. Furthermore, by constructing nanocapsules with similar sizes but different surface charges and chemistry, we demonstrated a generic strategy for prolonging the plasma half-life of therapeutic proteins. In an in vitro experiment, four types of bovine serum albumin (BSA) nanocapsules were incubated with fetal bovine serum (FBS) in phosphate buffer saline (PBS); the cell uptake by HeLa cells was monitored to systematically evaluate the characteristics of the surface chemistry during circulation. Single positron emission tomography–computed tomography (SPECT) was employed to allow real-time observation of the BSA nanoparticle distribution in vivo, as well as quantification of the plasma concentration after intravenous administration. This study offers a practical method for translating a broad range of proteins for clinical use.
Tsinghua University Press
Linlin Zhang,Yang Liu,Gan Liu,Duo Xu,Sheng Liang,Xinyuan Zhu,Yunfeng Lu,Hui Wang, Prolonging the plasma circulation of proteins by nano-encapsulation with phosphorylcholine-based polymer. NanoRes.2016, 9(8): 2424–2432