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Nano Research

Article Title

The influence of nanotopography on organelle organization and communication

Keywords

micro-/nanotopography, osteogenic differentiation, vesicle trafficking, organelle-specific GTPases, endoplasmic reticulumstress, unfolded protein response

Abstract

Cellular differentiation can be affected by the extracellular environment,particularly extracellular substrates. The nanotopography of the substrate maybe involved in the mechanisms of cellular differentiation in vivo. Organelles aremajor players in various cellular functions; however, the influence of nanotopographyon organelles has not yet been elucidated. In the present study, amicropit-nanotube topography (MNT) was fabricated on the titanium surface,and organelle-specific fluorescent probes were used to detect the intracellularorganelle organization of MG63 cells. Communication between organelles,identified by organelle-specific GTPase expression, was evaluated by quantitativepolymerase chain reaction and western blotting. Transmission electron microscopywas performed to evaluate the organelle structure. There were no significantdifferences in organelle distribution or number between the MNT and flatsurface. However, organelle-specific GTPases on the MNT were dramaticallydownregulated. In addition, obvious endoplasmic reticulum lumen dilationwas observed on the MNT surface, and the unfolded protein response (UPR)was also initiated. Regarding the relationships among organelle trafficking, UPR,and osteogenic differentiation, our findings may provide important insights intothe signal transduction induced by nanotopography.

Graphical Abstract

Publisher

Tsinghua University Press

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