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Nano Research

Article Title

Ferritin surplus in mouse spleen 14 months after intravenous injection of iron oxide nanoparticles at clinical dose

Authors

Alexandre Tamion, Institut Lumière Matière, UMR5306 Université Lyon 1- CNRS, Université de Lyon, 69622 Villeurbanne cedex, France
Matthias Hillenkamp, Institut Lumière Matière, UMR5306 Université Lyon 1- CNRS, Université de Lyon, 69622 Villeurbanne cedex, France
Arnaud Hillion, Institut Lumière Matière, UMR5306 Université Lyon 1- CNRS, Université de Lyon, 69622 Villeurbanne cedex, France
Valentin A. Maraloiu, National Institute of Materials Physics, 105bis Atomistilor St., R-077125 Bucharest-Magurele, Romania
Ioana D. Vlaicu, National Institute of Materials Physics, 105bis Atomistilor St., R-077125 Bucharest-Magurele, Romania
Mariana Stefan, National Institute of Materials Physics, 105bis Atomistilor St., R-077125 Bucharest-Magurele, Romania
Daniela Ghica, National Institute of Materials Physics, 105bis Atomistilor St., R-077125 Bucharest-Magurele, Romania
Hugo Rositi, CNRS, UMR 5220, INSERM, U1044, INSA de Lyon, Université de Lyon, Lyon 1, CREATIS, 69003 Lyon, France
Fabien Chauveau, CRNL-BIORAN, CNRS UMR5292, INSERM U1028, Université Lyon 1, 59 BD PINEL, 69677, Bron, France
Marie-Geneviève Blanchin, Institut Lumière Matière, UMR5306 Université Lyon 1- CNRS, Université de Lyon, 69622 Villeurbanne cedex, France
Marlène Wiart, CNRS, UMR 5220, INSERM, U1044, INSA de Lyon, Université de Lyon, Lyon 1, CREATIS, 69003 Lyon, France
Véronique Dupuis, Institut Lumière Matière, UMR5306 Université Lyon 1- CNRS, Université de Lyon, 69622 Villeurbanne cedex, France

Keywords

nanoparticles, biodegradation, iron oxide, nanomagnetism, contrast agent

Abstract

In this study, we followed the biodegradation of ultra-small superparamagneticiron oxide nanoparticles injected intravenously at clinical doses in mice.An advanced fitting procedure for magnetic susceptibility curves and lowtemperaturehysteresis loops was used to fully characterize the magnetic sizedistribution as well as the magnetic anisotropy energy of the injected P904 nanoparticles(Guerbet Laboratory). Additional magnetometry measurements andtransmission electronic microscopy observations were systematically performedto examine dehydrated samples from the spleen and liver of healthy C57B16mice after nanoparticle injection, with sacrifice of the mice for up to 14 months.At 3 months after injection, the magnetic properties of the spleen and liver weredramatically different. While the liver showed no magnetic signals other thanthose also present in the reference species, the spleen showed an increasedmagnetic signal attributed to ferritin. This surplus of ferritin remained constantup to 14 months after injection.

Graphical Abstract

Publisher

Tsinghua University Press

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