Ferritin surplus in mouse spleen 14 months after intravenous injection of iron oxide nanoparticles at clinical dose
nanoparticles, biodegradation, iron oxide, nanomagnetism, contrast agent
In this study, we followed the biodegradation of ultra-small superparamagneticiron oxide nanoparticles injected intravenously at clinical doses in mice.An advanced fitting procedure for magnetic susceptibility curves and lowtemperaturehysteresis loops was used to fully characterize the magnetic sizedistribution as well as the magnetic anisotropy energy of the injected P904 nanoparticles(Guerbet Laboratory). Additional magnetometry measurements andtransmission electronic microscopy observations were systematically performedto examine dehydrated samples from the spleen and liver of healthy C57B16mice after nanoparticle injection, with sacrifice of the mice for up to 14 months.At 3 months after injection, the magnetic properties of the spleen and liver weredramatically different. While the liver showed no magnetic signals other thanthose also present in the reference species, the spleen showed an increasedmagnetic signal attributed to ferritin. This surplus of ferritin remained constantup to 14 months after injection.
Tsinghua University Press
Alexandre Tamion,Matthias Hillenkamp,Arnaud Hillion,Valentin A. Maraloiu,Ioana D. Vlaicu,Mariana Stefan,Daniela Ghica,Hugo Rositi,Fabien Chauveau,Marie-Geneviève Blanchin,Marlène Wiart,Véronique Dupuis, Ferritin surplus in mouse spleen 14 months after intravenous injection of iron oxide nanoparticles at clinical dose. NanoRes.2016, 9(8): 2398–2410