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Nano Research

Article Title

Toxicity assessment and long-term three-photon fluorescence imaging of bright aggregation-induced emission nanodots in zebrafish

Authors

Dongyu Li, State Key Laboratory of Modern Optical Instrumentation, Centre for Optical and Electromagnetic Research, Zhejiang Provincial Key Laboratory for Sensing Technologies, Zhejiang University, Hangzhou 310058, China
Xinyuan Zhao, Bioelectromagnetics Laboratory, Institute of Environmental Health, Zhejiang University School of Medicine, Hangzhou 310058, China
Wei Qin, Department of Chemistry, The Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, Hong Kong, China
Hequn Zhang, State Key Laboratory of Modern Optical Instrumentation, Centre for Optical and Electromagnetic Research, Zhejiang Provincial Key Laboratory for Sensing Technologies, Zhejiang University, Hangzhou 310058, China
Yue Fei, Bioelectromagnetics Laboratory, Institute of Environmental Health, Zhejiang University School of Medicine, Hangzhou 310058, China
Liwei Liu, Changchun University of Science and Technology (CUST), Changchun 130022, China
Ken-Tye Yong, School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore 639798, Singapore
Guangdi Chen, Bioelectromagnetics Laboratory, Institute of Environmental Health, Zhejiang University School of Medicine, Hangzhou 310058, China
Ben Zhong Tang, Department of Chemistry, The Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, Hong Kong, China
Jun Qian, State Key Laboratory of Modern Optical Instrumentation, Centre for Optical and Electromagnetic Research, Zhejiang Provincial Key Laboratory for Sensing Technologies, Zhejiang University, Hangzhou 310058, China

Keywords

biocompatible organicnanodots, aggregation-inducedemission, three-photon fluorescence, bioimaging, zebrafish

Abstract

Aggregation-induced emission (AIE) luminogen displaaxys bright fluorescenceand has photobleaching resistance in its aggregation state. It is an ideal fluorescentcontrast agent for bioimaging. Multiphoton microscopy is an important tool forbioimaging since it possesses the ability to penetrate deep into biological tissues.Herein, we used AIE luminogen together with multiphoton microscopy forlong-term imaging of zebrafish. A typical AIE luminogen, 2,3-bis(4-(phenyl(4-(1,2,2-triphenylvinyl) phenyl)amino)phenyl) fumaronitrile (TPE-TPA-FN or TTF),was encapsulated with 1,2-distearoyl-sn-glycero-3-phosphoethanola-mine-N-[methoxy(polyethylene glycol)-2000] (DSPE-mPEG2000) to form nanodots thatexhibited bright three-photon fluorescence under 1,560 nm-femtosecond (fs)laser excitation. The TTF-nanodots were chemically stable in a wide range ofpH values and showed no in vivo toxicity in zebrafish according to a series ofbiological tests. The TTF-nanodots were microinjected into zebrafish embryos,and the different growth stages of the labeled embryos were monitored with athree-photon fluorescence microscope. TTF-nanodots could be traced inside thezebrafish body for as long as 120 hours. In addition, the TTF-nanodots wereutilized to target the blood vessel of zebrafish, and three-photon fluorescenceangiogram was performed. More importantly, these nanodots were highly resistantto photobleaching under 1,560 nm-fs excitation, allowing long-term imaging ofzebrafish.Nano ResearchDOI 10.1007/s12274-016-1084-zAddress correspondence to Guangdi Chen, chenguangdi

Graphical Abstract

Publisher

Tsinghua University Press

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