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Nano Research

Article Title

Integrin-targeting with peptide-bioconjugated semiconductor-magnetic nanocrystalline heterostructures

Authors

Gianpiero Valente, Università degli Studi di Bari Aldo Moro, Dipartimento di Chimica, Via Orabona 4, 70125 - Bari, Italy
Nicoletta Depalo, Istituto per i Processi Chimico-Fisici-CNR UOS Bari, Via Orabona 4, 70125 - Bari, Italy
Ivan de Paola, Istituto di Biostrutture e Bioimmagini-CNR, Via Mezzocannone, 16, 80134 - Napoli, Italy
Rosa Maria Iacobazzi, Università degli Studi di Bari Aldo Moro, Dipartimento di Farmacia – Scienze del Farmaco, Via Orabona 4, 70125 - Bari, Italy
Nunzio Denora, Università degli Studi di Bari Aldo Moro, Dipartimento di Farmacia – Scienze del Farmaco, Via Orabona 4, 70125 - Bari, Italy
Valentino Laquintana, Università degli Studi di Bari Aldo Moro, Dipartimento di Farmacia – Scienze del Farmaco, Via Orabona 4, 70125 - Bari, Italy
Roberto Comparelli, Istituto per i Processi Chimico-Fisici-CNR UOS Bari, Via Orabona 4, 70125 - Bari, Italy
Emiliano Altamura, Università degli Studi di Bari Aldo Moro, Dipartimento di Chimica, Via Orabona 4, 70125 - Bari, Italy
Tiziana Latronico, Università degli Studi di Bari Aldo Moro, Dipartimento di Bioscienze, Biotecnologie e Biofarmaceutica, Via Orabona 4, 70125 - Bari, Italy
Michele Altomare, Istituto per i Processi Chimico-Fisici-CNR UOS Bari, Via Orabona 4, 70125 - Bari, Italy
Elisabetta Fanizza, Università degli Studi di Bari Aldo Moro, Dipartimento di Chimica, Via Orabona 4, 70125 - Bari, Italy
Marinella Striccoli, Istituto per i Processi Chimico-Fisici-CNR UOS Bari, Via Orabona 4, 70125 - Bari, Italy
Angela Agostiano, Università degli Studi di Bari Aldo Moro, Dipartimento di Chimica, Via Orabona 4, 70125 - Bari, Italy Istituto per i Processi Chimico-Fisici-CNR UOS Bari, Via Orabona 4, 70125 - Bari, Italy
Michele Saviano, Istituto di Cristallografia-CNR, Via Amendola 122/O, 70126 Bari, Italy
Annarita Del Gatto, Istituto di Biostrutture e Bioimmagini-CNR, Via Mezzocannone, 16, 80134 - Napoli, Italy
Laura Zaccaro, Istituto di Biostrutture e Bioimmagini-CNR, Via Mezzocannone, 16, 80134 - Napoli, Italy
Maria Lucia Curri, Istituto per i Processi Chimico-Fisici-CNR UOS Bari, Via Orabona 4, 70125 - Bari, Italy

Keywords

nanocrystalline heterostructures, photoactive semiconductor, magnetic nanostructure, cyclic RGD peptide, αvβ3 integrin, active targeting

Abstract

Binary asymmetric nanocrystals (BNCs), composed of a photoactive TiO2 nanorod joined with a superparamagnetic γ-Fe2O3 spherical domain, were embedded in polyethylene glycol modified phospholipid micelle and successfully bioconjugated to a suitably designed peptide containing an RGD motif. BNCs represent a relevant multifunctional nanomaterial, owing to the coexistence of two distinct domains in one particle, characterized by high photoactivity and magnetic properties, that is particularly suited for use as a phototherapy and hyperthermia agent as well as a magnetic probe in biological imaging. We selected the RGD motif in order to target integrin expressed on activated endothelial cells and several types of cancer cells. The prepared RGD-peptide/BNC conjugates, comprehensively monitored by using complementary optical and structural techniques, demonstrated a high stability and uniform dispersibility in biological media. The cytotoxicity of the RGD-peptide/BNC conjugates was studied in vitro. The cellular uptake of RGD-peptide conjugates in the cells, assessed by means of two distinct approaches, namely confocal microscopy analysis and emission spectroscopy determination in cell lysates, displayed selectivity of the RGD-peptide-BNC conjugate for the αvβ3 integrin. These RGD-peptide-BNC conjugates have a high potential for theranostic treatment of cancer.

Graphical Abstract

Publisher

Tsinghua University Press

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