Size-dependent gene delivery of amine-modified silica nanoparticles
silica nanoparticles, gene delivery, plasmid DNA, particle sizes, cellular uptake
ABSTRACT Silica-based nanoparticles are promising carriers for gene delivery applications. To gain insights into the effect of particle size on gene transfection efficiency, amine-modified monodisperse Stöber spheres (NH2-SS) with diameters of 125, 230, 330, 440, and 570 nm were synthesized. The in vitro transfection efficiencies of NH2-SS for delivering plasmid DNA encoding green fluorescent protein (GFP) (pcDNA3-EGFP, abbreviated as pcDNA, 6.1 kbp) were studied in HEK293T cells. NH2-SS with a diameter of 330 nm (NH2-SS330) showed the highest GFP transfection level compared to NH2-SS particles with other sizes. The transfection efficiency was found as a compromise between the binding capacity and cellular uptake performance of NH2-SS330 and pcDNA conjugates. NH2-SS330 also demonstrated the highest transfection efficiency for plasmid DNA (pDNA) with a bigger size of 8.9 kbp. To our knowledge, this study is the first to demonstrate the significance of particle size for gene transfection efficiency in silica-based gene delivery systems. Our findings are crucial to the rational design of synthetic vectors for gene therapy.
Tsinghua University Press
Meihua Yu,Yuting Niu,Jun Zhang,Hongwei Zhang,Yannan Yang,Elena Taran,Siddharth Jambhrunkar,Wenyi Gu,Peter Thorn,Chengzhong Yu, Size-dependent gene delivery of amine-modified silica nanoparticles. NanoRes.2016, 9(2): 291–305