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Nano Research

Article Title

Upconversion nano-photosensitizer targeting into mitochondria for cancer apoptosis induction and cyt c fluorescence monitoring

Authors

Yanyan Liu, State Key Laboratory of High Performance Ceramics and Superfine Microstructures, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai 200050, China
Jiawen Zhang, Department of Radiology, Huashan Hospital, Fudan University, 12 Urumqi Zhong Road, Shanghai 200040, China
Changjing Zuo, Department of Nuclear Medicine, Changhai Hospital of Shanghai, 168 Changhai Road, Shanghai 200433, China
Zhen Zhang, Cancer Research Institute, Fudan University Shanghai Cancer Center, 270 Dongan Road, Shanghai 200032, China
Dalong Ni, State Key Laboratory of High Performance Ceramics and Superfine Microstructures, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai 200050, China
Chen Zhang, State Key Laboratory of High Performance Ceramics and Superfine Microstructures, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai 200050, China
Jing Wang, Department of Radiology, Huashan Hospital, Fudan University, 12 Urumqi Zhong Road, Shanghai 200040, China
Hui Zhang, Cancer Research Institute, Fudan University Shanghai Cancer Center, 270 Dongan Road, Shanghai 200032, China
Zhenwei Yao, Department of Radiology, Huashan Hospital, Fudan University, 12 Urumqi Zhong Road, Shanghai 200040, China
Wenbo Bu, State Key Laboratory of High Performance Ceramics and Superfine Microstructures, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai 200050, China Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, 3663 Zhongshan North Road, Shanghai 200062, China

Keywords

mitochondrial reactive oxygen species (mitoROS), upconversion-based photodynamic therapy (PDT), cytochrome c (cyt c), mitochondrial apoptosis

Abstract

ABSTRACT Disruption of mitochondrial reactive oxygen species (mitoROS) plays a major role in cancer cell apoptosis. Here, we designed a core/shell-structured mitochondriatargeting upconversion-based nano-photosensitizer (TPP-UC(PS)) with a lanthanidedoped upconversion nanoparticle (UCNP) core coated by a photosensitizer (PS)-incorporated dense silica shell. Following irradiation with external nearinfrared laser (NIR), TPP-UC(PS) in mitochondria caused serious mitochondrial matrix swelling for the activated upconversion-based photodynamic therapy (UC-PDT), and the mobilization of cytochrome c (cyt c) was amplified in response to the increased mitoROS. Specifically, this heme-containing cyt c could be monitored by varying TPP-UC(PS)’s upconversion luminescence signal (UCL), which may facilitate the in situ detection of cyt c for apoptosis research. As a proof of concept, our designed TPP-UC(PS) may provide significant opportunities for controlling cancer cell apoptosis under NIR stimulation and for studying apoptosis using the dynamic UCL, which is influenced by local cyt c.

Graphical Abstract

Publisher

Tsinghua University Press

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