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Nano Research

Article Title

Aggregation-induced emission (AIE) dye loaded polymer nanoparticles for gene silencing in pancreatic cancer and their in vitro and in vivo biocompatibility evaluation

Authors

Rui Hu, School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore 639798, Singapore
Chengbin Yang, School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore 639798, Singapore
Yucheng Wang, School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore 639798, Singapore
Guimiao Lin, The Engineering Lab of Synthetic Biology and the Key Lab of Biomedical Engineering, School of Medicine, Shenzhen University,Shenzhen, 518060, China
Wei Qin, Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
Qingling Ouyang, School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore 639798, Singapore
Wing-Cheung Law, Department of Industrial and Systems Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, P.R. China
Quoc Toan Nguyen, Division of Structural Biology & Biochemistry, School of Biological Sciences, Nanyang Technological University, Singapore 639798,Singapore
Ho Sup Yoon, Division of Structural Biology & Biochemistry, School of Biological Sciences, Nanyang Technological University, Singapore 639798,Singapore
Xiaomei Wang, The Engineering Lab of Synthetic Biology and the Key Lab of Biomedical Engineering, School of Medicine, Shenzhen University,Shenzhen, 518060, China
Ken-Tye Yong, School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore 639798, Singapore
Ben Zhong Tang, Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China

Keywords

aggregation-induced emission, polymer nanoparticles, gene silencing, pancreatic cancer

Abstract

We have developed aggregation-induced emission (AIE) dye loaded polymer nanoparticles with deep-red emission for siRNA delivery to pancreatic cancer cells. Two US Food and Drug Administration (FDA) approved surfactant polymers, Pluronics F127 and PEGylated phospholipid, were used to prepare the dye-loaded nanoparticle formulations and they can be used as nanovectors for gene silencing of mutant K-ras in pancreatic cancer cells. The successful transfection of siRNA by the developed nanovectors was confirmed by the fluorescent imaging and quantified through flow cytometry. Quantitative real time polymerase chain reaction (PCR) indicates that the expression of the mutant K-ras oncogene from the MiaPaCa-2 pancreatic cancer cells has been successfully suppressed. More importantly, our in vivo toxicity study has revealed that both the nanoparticle formulations are highly biocompatible in BALC/c mice. Overall, our results suggest that the AIE dye-loaded polymer nanoparticle formulations developed here are suitable for gene delivery and have high potential applications in translational medicine research.

Graphical Abstract

Publisher

Tsinghua University Press

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