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Nano Research

Article Title

1.3 μm emitting SrF2:Nd3+ nanoparticles for high contrast in vivo imaging in the second biological window

Authors

Irene Villa, Department of Materials Science, University of Milano-Bicocca, Via R. Cozzi 55 , 20125 Milano, Italy
Anna Vedda, Department of Materials Science, University of Milano-Bicocca, Via R. Cozzi 55 , 20125 Milano, Italy
Irene Xochilt Cantarelli, Dipartimento di Biotecnologie, Università di Verona and INSTM, UdR Verona, Strada le Grazie 15, 37134 Verona, Italy
Marco Pedroni, Dipartimento di Biotecnologie, Università di Verona and INSTM, UdR Verona, Strada le Grazie 15, 37134 Verona, Italy
Fabio Piccinelli, Dipartimento di Biotecnologie, Università di Verona and INSTM, UdR Verona, Strada le Grazie 15, 37134 Verona, Italy
Marco Bettinelli, Dipartimento di Biotecnologie, Università di Verona and INSTM, UdR Verona, Strada le Grazie 15, 37134 Verona, Italy
Adolfo Speghini, Dipartimento di Biotecnologie, Università di Verona and INSTM, UdR Verona, Strada le Grazie 15, 37134 Verona, Italy
Marta Quintanilla, Institut National de la Recherche Scientifique-Energie Materiaux et Telecommunications, Université du Quebec, Varennes, QC, PQ J3X 1S2, Canada
Fiorenzo Vetrone, Institut National de la Recherche Scientifique-Energie Materiaux et Telecommunications, Université du Quebec, Varennes, QC, PQ J3X 1S2, Canada
Ueslen Rocha, Grupo de Fotnica e Fluidos Complexos, Instituto de Física, Universidade Federal de Alagoas, 57072-970, Maceió, Alagoas, Brazil
Carlos Jacinto, Grupo de Fotnica e Fluidos Complexos, Instituto de Física, Universidade Federal de Alagoas, 57072-970, Maceió, Alagoas, Brazil
Elisa Carrasco, Departamento de Biología, Facultad de Ciencias, Campus de Cantoblanco, Universidad Autónoma de Madrid, Madrid, 28049, Spain
Francisco Sanz Rodríguez, Departamento de Biología, Facultad de Ciencias, Campus de Cantoblanco, Universidad Autónoma de Madrid, Madrid, 28049, Spain
ngeles Juarranz, Departamento de Biología, Facultad de Ciencias, Campus de Cantoblanco, Universidad Autónoma de Madrid, Madrid, 28049, Spain
Blanca del Rosal, Fluorescence Imaging Group, Departamento de Física de Materiales, Facultad de Ciencias, Campus de Cantoblanco, Universidad Autónoma de Madrid, Madrid 28049, Spain
Dirk H. Ortgies, Fluorescence Imaging Group, Departamento de Física de Materiales, Facultad de Ciencias, Campus de Cantoblanco, Universidad Autónoma de Madrid, Madrid 28049, Spain
Patricia Haro Gonzalez, Fluorescence Imaging Group, Departamento de Física de Materiales, Facultad de Ciencias, Campus de Cantoblanco, Universidad Autónoma de Madrid, Madrid 28049, Spain
José García Solé, Fluorescence Imaging Group, Departamento de Física de Materiales, Facultad de Ciencias, Campus de Cantoblanco, Universidad Autónoma de Madrid, Madrid 28049, Spain
Daniel Jaque García, Fluorescence Imaging Group, Departamento de Física de Materiales, Facultad de Ciencias, Campus de Cantoblanco, Universidad Autónoma de Madrid, Madrid 28049, Spain

Keywords

fluorescence imaging, rare earth doped nanoparticles, nanomedicine

Abstract

Novel approaches for high contrast, deep tissue, in vivo fluorescence biomedical imaging are based on infrared-emitting nanoparticles working in the so-called second biological window (1,000–1,400 nm). This allows for the acquisition of high resolution, deep tissue images due to thepartial transparency of tissues in this particular spectral range. In addition, the optical excitation with low energy (infrared) photons also leads to a drastic reduction in the contribution of autofluorescence to the in vivo image. Nevertheless, as is demonstrated here, working solely in this biological window does not ensure a complete removal of autofluorescence as the specimen’s diet shows a remarkable infrared fluorescence that extends up to 1,100 nm. In this work, we show how the 1,340 nm emission band of Nd3+ ions embedded in SrF2 nanoparticles can be used to produce autofluorescence free, high contrast in vivo fluorescence images. It is also dem- onstrated that the complete removal of the food-related infrared autofluorescence is imperative for the development of reliable biodistribution studies.

Graphical Abstract

Publisher

Tsinghua University Press

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