Article Title

Aqueous self-assembly and surface-functionalized nanodots for live cell imaging and labeling

Authors

Mei-Lang Kung, Department of Chemistry and Center for Nanoscience and Nanotechnology, “National Sun Yat-sen University”, Kaohsiung 80424
Pei-Ying Lin, Department of Chemistry and Center for Nanoscience and Nanotechnology, “National Sun Yat-sen University”, Kaohsiung 80424
Chiung-Wen Hsieh, Department of Chemistry and Center for Nanoscience and Nanotechnology, “National Sun Yat-sen University”, Kaohsiung 80424
Shuchen Hsieh, Department of Chemistry and Center for Nanoscience and Nanotechnology, “National Sun Yat-sen University”, Kaohsiung 80424 School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80707

Keywords

atomic force microscopy, hydrophilicity, molecular imaging, self-assembly, silane

Abstract

Nanoparticles have enormous potential for bioimaging and biolabeling applications, in which conventional organically based fluorescent labels degrade and fail to provide long-term tracking. Thus, the development of approaches to make fluorescent probes water soluble and label cells efficient is desirable for most biological applications. Here, we report on the fabrication and charac- terization of self-assembled nanodots (SANDs) from 3-aminopropyltriethoxysilane (APTES) as a probe for protein labeling. We show that fluorescent SAND probes exhibit both bright photoluminescence and biocompatibility in an aqueous environment. Selective in vitro imaging using protein and carbohydrate labeling of hepatoma cell lines are demonstrated using biocompatible SANDs conjugated with avidin and galactose, respectively. Cytotoxicity tests show that conjugated SAND particles have negligible effects on cell proliferation. Unlike other synthetic systems that require multistep treatments to achieve robust surface functionalization and to develop flexible bioconjugation strategies, our results demonstrate the versatility of this one-step SAND fabrication method for creating multicolor fluorescent probes with the tailored functionalities, efficient emission, as well as excellent biocompatibility, required for broad biological use.

Graphical Abstract

DOI

https://doi.org/10.1007/s12274-014-0479-y

Publisher

Tsinghua University Press

Recommended Citation

Mei-Lang Kung,Pei-Ying Lin,Chiung-Wen Hsieh,Shuchen Hsieh, Aqueous self-assembly and surface-functionalized nanodots for live cell imaging and labeling. NanoRes.2014, 7(8): 1164–1176