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Nano Research

Article Title

Large-scale dendrimer-based uneven nanopatterns for the study of local arginine-glycine-aspartic acid (RGD) density effects on cell adhesion

Authors

Anna Lagunas, Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain Institute for Bioengineering of Catalonia (IBEC), Baldiri-Reixac 15-21, Barcelona 08028, Spain
Albert G. Castao, Institute for Bioengineering of Catalonia (IBEC), Baldiri-Reixac 15-21, Barcelona 08028, Spain Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain
Juan M. Artés, Institute for Bioengineering of Catalonia (IBEC), Baldiri-Reixac 15-21, Barcelona 08028, Spain Physical Chemistry Department, University of Barcelona (UB), Martí i Franquès 1-11, Barcelona 08028, Spain
Yolanda Vida, Andalusian Centre for Nanomedicine and Biotechnology (BIONAND), Severo Ochoa 35, Málaga 29590, Spain Organic Chemistry Department, University of Málaga (UMA), Campus Teatinos, Málaga 29071, Spain
Daniel Collado, Andalusian Centre for Nanomedicine and Biotechnology (BIONAND), Severo Ochoa 35, Málaga 29590, Spain Organic Chemistry Department, University of Málaga (UMA), Campus Teatinos, Málaga 29071, Spain
Ezequiel Pérez-Inestrosa, Andalusian Centre for Nanomedicine and Biotechnology (BIONAND), Severo Ochoa 35, Málaga 29590, Spain Organic Chemistry Department, University of Málaga (UMA), Campus Teatinos, Málaga 29071, Spain
Pau Gorostiza, Institute for Bioengineering of Catalonia (IBEC), Baldiri-Reixac 15-21, Barcelona 08028, Spain Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain Institució Catalana de Recerca i Estudis Avanats (ICREA), Spain
Silvia Claros, Cell Biology, Genetics and Physiology Department, University of Málaga (UMA), Campus Teatinos, Málaga 29071, Spain Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain
José A. Andrades, Cell Biology, Genetics and Physiology Department, University of Málaga (UMA), Campus Teatinos, Málaga 29071, Spain Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain
Josep Samitier, Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain Institute for Bioengineering of Catalonia (IBEC), Baldiri-Reixac 15-21, Barcelona 08028, Spain Electronics Department, University of Barcelona (UB), Martí i Franquès 1-11, Barcelona 08028, Spain

Keywords

dendrimer, arginine-glycine-aspartic acid, atomic force microscopy, scanning tunneling microscopy, cell adhesion, focal adhesions

Abstract

Cell adhesion processes are governed by the nanoscale arrangement of the extracellular matrix (ECM), being more affected by local rather than global concentrations of cell adhesive ligands. In many cell-based studies, grafting of dendrimers on surfaces has shown the benefits of the local increase in concentration provided by the dendritic configuration, although the lack of any reported surface characterization has limited any direct correlation between dendrimer disposition and cell response. In order to establish a proper correlation, some control over dendrimer surface deposition is desirable. Here, dendrimer nanopatterning has been employed to address arginine–glycine–aspartic acid (RGD) density effects on cell adhesion. Nanopatterned surfaces were fully characterized by atomic force microscopy (AFM), scanning tunneling microscopy (STM) and X-ray photoelectron spectroscopy (XPS), showing that tunable distributions of cell adhesive ligands on the surface are obtained as a function of the initial dendrimer bulk concentration. Cell experiments showed a clear correlation with dendrimer surface layout: Substrates presenting regions of high local ligand density resulted in a higher percentage of adhered cells and a higher degree of maturation of focal adhesions (FAs). Therefore, dendrimer nanopatterning is presented as a suitable and controlled approach to address the effect of local ligand density on cell response. Moreover, due to the easy modification of dendrimer peripheral groups, dendrimer nanopatterning can be further extended to other ECM ligands having density effects on cells.

Graphical Abstract

Publisher

Tsinghua University Press

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