Article Title

In situ generated thrombin in the protein corona of zeolites: Relevance of the functional proteins to its biological impact

Authors

Yunlong Li, Key Lab of Applied Chemistry of Zhejiang Province, Zhejiang University, Hangzhou 310027, China
Xiaofeng Liao, Key Lab of Applied Chemistry of Zhejiang Province, Zhejiang University, Hangzhou 310027, China
Xiaoxi Zhang, Department of Chemistry, Fudan University, Shanghai 200433, China
Guicen Ma, Key Lab of Applied Chemistry of Zhejiang Province, Zhejiang University, Hangzhou 310027, China
Shuai Zuo, Department of Chemistry, Fudan University, Shanghai 200433, China
Liping Xiao, Key Lab of Applied Chemistry of Zhejiang Province, Zhejiang University, Hangzhou 310027, China
Galen D. Stucky, Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA 93106, USA
Zhugang Wang, Department of Medical Genetics, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
Xian Chen, Department of Chemistry, Fudan University, Shanghai 200433, China Department of Biochemistry and Biophysics, School of Medicine, University of North Carolina-Chapel Hill, NC 27599, USA
Xiaoqiang Shang, Key Lab of Applied Chemistry of Zhejiang Province, Zhejiang University, Hangzhou 310027, China
Jie Fan, Key Lab of Applied Chemistry of Zhejiang Province, Zhejiang University, Hangzhou 310027, China

Keywords

protein corona, zeolite, thrombin activity, procoagulant activity, calcium

Abstract

Adsorption of plasma proteins to nanomaterial surfaces has a great influence on their bio-functionality. However, there is limited understanding of the relationship between the functional proteins in the protein corona and the biological identity of the materials. Here we show that the in situ generated thrombin in the protein corona of a Ca-zeolite surface displays a calcium-dependent, unusually high (~3,000 NIH U/mg) procoagulant activity, which is even stable against antithrombin deactivation. Removing the encapsulated Ca2+ in the zeolites leads to deactivation by antithrombin. Our observations suggest that the thrombin activity can be regulated by the inorganic surface and cations. Most importantly, our discovery indicates the link between the biomolecules in the protein corona and the procoagulant activity of the materials, providing a new molecular basis for the procoagulant mechanism for zeolite hemostatics.

Graphical Abstract

DOI

https://doi.org/10.1007/s12274-014-0505-0

Publisher

Tsinghua University Press

Recommended Citation

Yunlong Li,Xiaofeng Liao,Xiaoxi Zhang,Guicen Ma,Shuai Zuo,Liping Xiao,Galen D. Stucky,Zhugang Wang,Xian Chen,Xiaoqiang Shang,Jie Fan, In situ generated thrombin in the protein corona of zeolites: Relevance of the functional proteins to its biological impact. NanoRes.2014, 7(10): 1457–1465