Article Title

Functionalization of high-moment magnetic nanodisks for cell manipulation and separation

Authors

Mingliang Zhang, 476 Lomita Mall, Department of Materials Science and Engineering, Stanford University, Stanford 94305, USA
Christopher M. Earhart, 476 Lomita Mall, Department of Materials Science and Engineering, Stanford University, Stanford 94305, USA
Chinchun Ooi, 476 Lomita Mall, Department of Materials Science and Engineering, Stanford University, Stanford 94305, USA
Robert J. Wilson, 476 Lomita Mall, Department of Materials Science and Engineering, Stanford University, Stanford 94305, USA
Mary Tang, 350 Serra Mall, Department of Electrical Engineering, Stanford University, Stanford 94305, USA
Shan X. Wang, 476 Lomita Mall, Department of Materials Science and Engineering, Stanford University, Stanford 94305, USA 350 Serra Mall, Department of Electrical Engineering, Stanford University, Stanford 94305, USA

Keywords

cell separation, core–shell, functionalization, magnetic properties, nanotechnology

Abstract

Synthetic antiferromagnetic (SAF) nanoparticles are layer-structured particles with high single-particle magnetic moments. In order to covalently bind these nanoparticles to cells, they were coated with a silica shell followed by conjugation with streptavidin. The silica coating generates both SAF@SiO2 core–shell nanoparticles and silica core-free nanoparticles. Using a simple magnetic separation, silica nanoparticles were removed and SAF@SiO2 nanoparticles were purified. After streptavidin conjugation, these particles were used to stain lung cancer cells, making them highly magnetically responsive. The stained cells can rotate in response to an external magnetic field and can be captured when a blood sample containing these cells flows through the sifter.

Graphical Abstract

DOI

https://doi.org/10.1007/s12274-013-0352-4

Publisher

Tsinghua University Press

Recommended Citation

Mingliang Zhang,Christopher M. Earhart,Chinchun Ooi,Robert J. Wilson,Mary Tang,Shan X. Wang, Functionalization of high-moment magnetic nanodisks for cell manipulation and separation. NanoRes.2013, 6(10): 745–751