Easy access to selective binding and recyclable separation of histidine-tagged proteins using Ni2+-decorated superparamagnetic nanoparticles
Magnetic nanoparticles, green synthesis, protein purification, recyclable process, biotechnology
ABSTRACT The development of simple techniques for the separation and purification of recombinant proteins plays an important role in many of the advancements made in biotechnology and nanotechnology. Herein, we report an easy method for the efficient purification of polyhistidine affinity-tagged (His-tagged) proteins by using Ni2+-decorated superparamagnetic particles. Monodisperse Ni0.3Fe0.7Fe2O4 nanoparticles were prepared via a facile and economical one-pot hydrothermal process. Owing to the characteristic molecular recognition ability between nickel(Ⅱ) ions and the polyhistidine affinity tag, the nanoparticles could be successfully employed to selectively bind and separate His-tagged cyan fluorescent protein (CFP) from an E. coli cell lysate in a recyclable process. Moreover, by changing the divalent metal precursors, various other metal-decorated magnetic nanoparticles can be obtained. This approach offers the possibility of constructing metal-decorated nanoparticles through a simple method and will be highly beneficial in further applications of nanoparticlebased technologies.
Tsinghua University Press
Zhen Liu,Meng Li,Zhenhua Li,Fang Pu,Jinsong Ren,Xiaogang Qu, Easy access to selective binding and recyclable separation of histidine-tagged proteins using Ni2+-decorated superparamagnetic nanoparticles. NanoRes.2012, 5(7): 450–459