Fluorophore-photochrome co-embedded polymer nanoparticles for photoswitchable fluorescence bioimaging
Photoswithable imaging, polymer nanoparticles, fluorophore–photochrome, living cell
ABSTRACT Photoswitchable polymer nanoparticles (ps-PNPs) have been constructed by emulsion polymerization through embedding an iridium(Ⅲ) complex, [Ir(bt)2(pic)] (bt = phenylbenzothiazole, pic = picolinate), as a fluorophore and a diarylethene derivative (DTE) as a photochrome. The ps-PNPs show reversible luminescence modulation with DTE switching using UV light in aqueous solution and even in living cells. Such luminescent modulation only occurred in polymer nanoparticles and not in a simple mixed solution with low concentrations of [Ir(bt)2(pic)] and DTE. The ps-PNPs displayed good cellular uptake and fast intracellular luminescence modulation by photoswitching, indicating the nanoparticles could be developed as photoswitchable luminescent probes for a diversity of bioimaging applications.
Tsinghua University Press
Tianshe Yang,Qian Liu,Shouzhi Pu,Zesheng Dong,Chunhui Huang,Fuyou Li, Fluorophore-photochrome co-embedded polymer nanoparticles for photoswitchable fluorescence bioimaging. NanoRes.2012, 5(7): 494–503