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Nano Research

Article Title

A facile assay for direct colorimetric visualization of lipopolysaccharides at low nanomolar level

Authors

Jiayu Sun, Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China
Jiechao Ge, Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China
Weimin Liu, Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China
Xueliang Wang, Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China
Zhiyuan Fan, Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China
Wenwen Zhao, Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China
Hongyan Zhang, Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China
Pengfei Wang, Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China
Shuit-Tong Lee, Center of Super-Diamond and Advanced Films (COSDAF) and Department of Physics and Materials Science, City University of Hong Kong, Hong Kong, China

Keywords

lipopolysaccharide (LPS), endotoxin, Gram-negative, sensor, gold nanoparticle

Abstract

ABSTRACT We report a facile assay for the rapid visual detection of lipopolysaccharide (LPS) molecules down to the low nanomolar level by taking advantage of the electrostatic interaction between LPS molecules and cysteaminemodified gold nanoparticles (CSH–Au NPs). The large amount of negatively charged groups on the LPS molecules make LPS highly negatively charged. Thus, when modified with cysteamine, the positively charged gold nanoparticles can aggregate in the presence of trace amounts of LPS. The probe is simple, does not require any advanced instrumentation, and the limit of detection (LOD) was determined to be as low as 3.3 × 10–10 mol/L. To the best of our knowledge, it is the most sensitive synthetic LPS sensor reported so far.

Graphical Abstract

Publisher

Tsinghua University Press

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