One-pot polymerase chain reaction with gold nanoparticles for rapid and ultrasensitive DNA detection
deoxyribonucleic acid (DNA) detection, polymerase chain reaction (PCR), Au nanoparticle, colorimetric
We report a novel method for rapid, colorimetric detection of a specific deoxyribonucleic acid (DNA) sequence by carrying out a polymerase chain reaction in the presence of gold nanoparticles functionalized with two primers. Extension of the primers when the target DNA is present as a template during the polymerase chain reaction process affords the complementary sequences on the gold nanoparticle surfaces and results in the formation of gold nanoparticle aggregates with a concomitant color change from red to pinkish/purple. This method provides a convenient and straightforward solution for ultrasensitive DNA detection without any further post-treatment of the polymerase chain reaction products being necessary, and is a promising tool for rapid disease diagnostics and gene sequencing.
Tsinghua University Press
Miao Cai,Feng Li,Yan Zhang,Qiangbin Wang, One-pot polymerase chain reaction with gold nanoparticles for rapid and ultrasensitive DNA detection. NanoRes.2010, 3: 557–563