Article Title

One-pot polymerase chain reaction with gold nanoparticles for rapid and ultrasensitive DNA detection

Authors

Miao Cai, i-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China
Feng Li, i-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China
Yan Zhang, i-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China
Qiangbin Wang, i-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China

Keywords

deoxyribonucleic acid (DNA) detection, polymerase chain reaction (PCR), Au nanoparticle, colorimetric

Abstract

We report a novel method for rapid, colorimetric detection of a specific deoxyribonucleic acid (DNA) sequence by carrying out a polymerase chain reaction in the presence of gold nanoparticles functionalized with two primers. Extension of the primers when the target DNA is present as a template during the polymerase chain reaction process affords the complementary sequences on the gold nanoparticle surfaces and results in the formation of gold nanoparticle aggregates with a concomitant color change from red to pinkish/purple. This method provides a convenient and straightforward solution for ultrasensitive DNA detection without any further post-treatment of the polymerase chain reaction products being necessary, and is a promising tool for rapid disease diagnostics and gene sequencing.

Graphical Abstract

DOI

https://doi.org/10.1007/s12274-010-0016-6

Publisher

Tsinghua University Press

Recommended Citation

Miao Cai,Feng Li,Yan Zhang,Qiangbin Wang, One-pot polymerase chain reaction with gold nanoparticles for rapid and ultrasensitive DNA detection. NanoRes.2010, 3: 557–563