•  
  •  
 
Nano Research

Article Title

Pullulan acetate coated magnetite nanoparticles for hyper-thermia: Preparation, characterization and in vitro experiments

Authors

Fuping Gao, Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, Beijing 100084, China
Yuanyuan Cai, Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, Beijing 100084, China Department of Biopharmaceutics, Beijing University of Traditional Chinese Medicine, Beijing 100102, China
Jing Zhou, Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, Beijing 100084, China Department of Biopharmaceutics, Beijing University of Traditional Chinese Medicine, Beijing 100102, China
Xiaoxue Xie, Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, Beijing 100084, China Department of Oncology, Xiangya Hospital of Central South University, Changsha 410008, China
Weiwei Ouyang, Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, Beijing 100084, China Department of Oncology, Xiangya Hospital of Central South University, Changsha 410008, China
Yuhui Zhang, Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, Beijing 100084, China Department of Biopharmaceutics, Beijing University of Traditional Chinese Medicine, Beijing 100102, China
Xufei Wang, Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, Beijing 100084, China
Xiaodong Zhang, Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, Beijing 100084, China
Xiaowen Wang, Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, Beijing 100084, China
Lingyun Zhao, Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, Beijing 100084, China
Jintian Tang, Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, Beijing 100084, China

Keywords

Magnetic nanoparticles, pullulan acetate, hyperthermia, cytotoxicity, cellular uptake

Abstract

Amphipathic polymer pullulan acetate (PA)-coated magnetic nanoparticles were prepared and characterized by various physicochemical means. The cytotoxicity and cellular uptake of the magnetic nanoparticles were examined. The hyperthermic effect of the magnetic nanoparticles on tumor cells was evaluated. Transmission electron microscopy (TEM) showed that the PA coated magnetic nanoparticles (PAMNs) had spherical morphology. Dynamic light scattering (DLS) showed that the size distribution of PAMNs was unimodal,with an average diameter of 25.8 nm ± 6.1 nm. The presence of the adsorbed layer of PA on the magnetite surface was confirmed by Fourier transform infrared (FTIR) spectroscopy. Magnetic measurements revealed that the saturation magnetization of the PAMNs reached 51.9 emu/g and the nanoparticles were superparamagnetic. Thermogravimetric analysis (TGA) showed that the Fe3O4 particles constituted 75 wt% of the PAMNs. The PAMNs had good heating properties in an alternating magnetic field. Cytotoxicity assay showed that PAMNs exhibited no significant cytotoxicity against L929 cells. TEM results showed that a large number of PAMNs were internalized into KB cells. PAMNs have good hyperthermia effect on KB cells in vitro by magnetic field induced hyperthermia. These novel magnetic nanoparticles have great potential as magnetic hyperthermia mediators.

Graphical Abstract

Publisher

Tsinghua University Press

Share

COinS