•  
  •  
 
Nano Research

Article Title

Functionalization of single- and multi-walled carbon nanotubes with cationic amphiphiles for plasmid DNA complexation and transfection

Authors

Cyrille Richard, Unité de Pharmacologie Chimique et Génétique,CNRS, UMR 8151, Paris, F-75270 cedex France INSERM, U 640, Paris, F-75270 cedex France Université Paris Descartes, Faculté des Sciences Pharmaceutiques et Biologiques, Paris, F-75270 cedex France ENSCP, Paris, F-75231 cedex France
Nathalie Mignet, Unité de Pharmacologie Chimique et Génétique,CNRS, UMR 8151, Paris, F-75270 cedex France INSERM, U 640, Paris, F-75270 cedex France Université Paris Descartes, Faculté des Sciences Pharmaceutiques et Biologiques, Paris, F-75270 cedex France ENSCP, Paris, F-75231 cedex France
Céline Largeau, Unité de Pharmacologie Chimique et Génétique,CNRS, UMR 8151, Paris, F-75270 cedex France INSERM, U 640, Paris, F-75270 cedex France Université Paris Descartes, Faculté des Sciences Pharmaceutiques et Biologiques, Paris, F-75270 cedex France ENSCP, Paris, F-75231 cedex France
Virginie Escriou, Unité de Pharmacologie Chimique et Génétique,CNRS, UMR 8151, Paris, F-75270 cedex France INSERM, U 640, Paris, F-75270 cedex France Université Paris Descartes, Faculté des Sciences Pharmaceutiques et Biologiques, Paris, F-75270 cedex France ENSCP, Paris, F-75231 cedex France
Michel Bessodes, Unité de Pharmacologie Chimique et Génétique,CNRS, UMR 8151, Paris, F-75270 cedex France INSERM, U 640, Paris, F-75270 cedex France Université Paris Descartes, Faculté des Sciences Pharmaceutiques et Biologiques, Paris, F-75270 cedex France ENSCP, Paris, F-75231 cedex France
Daniel Scherman, Unité de Pharmacologie Chimique et Génétique,CNRS, UMR 8151, Paris, F-75270 cedex France INSERM, U 640, Paris, F-75270 cedex France Université Paris Descartes, Faculté des Sciences Pharmaceutiques et Biologiques, Paris, F-75270 cedex France ENSCP, Paris, F-75231 cedex France

Keywords

Carbon nanotubes, amphiphile, gene delivery, plasmid DNA, in vitro

Abstract

The possibility of delivering DNA effi ciently to cells represents a crucial issue for the treatment of both genetic and acquired diseases. However, even although the effi ciency of non-viral transfection systems has improved in the last decade, none have yet proven to be suffi ciently effective in vivo. We report herein our results on the functionalization of single-walled carbon nanotubes (SWNT) and multi-walled carbon nanotubes (MWNT) by two cationic amphiphiles (lipid RPR120535 and pyrenyl polyamine), their use for the complexation of plasmid DNA, and their efficiency in transfecting cells in vitro. The experiments have shown that the efficiency of transfection is higher when using SWNT instead of MWNT, and that transfection effi ciency is similar or slightly higher when using nanoplexes (SWNT/lipid RPR120535/DNA) instead of lipoplexes (lipid RPR120535/DNA) and several orders of magnitude higher than that of naked DNA. This study therefore shows both that the transfection is better when using SWNTs and that it is dependent on the nature of the amphiphilic molecules adsorbed on the nanotubes.

Graphical Abstract

Publisher

Tsinghua University Press

Share

COinS