Functionalization of single- and multi-walled carbon nanotubes with cationic amphiphiles for plasmid DNA complexation and transfection
Carbon nanotubes, amphiphile, gene delivery, plasmid DNA, in vitro
The possibility of delivering DNA effi ciently to cells represents a crucial issue for the treatment of both genetic and acquired diseases. However, even although the effi ciency of non-viral transfection systems has improved in the last decade, none have yet proven to be suffi ciently effective in vivo. We report herein our results on the functionalization of single-walled carbon nanotubes (SWNT) and multi-walled carbon nanotubes (MWNT) by two cationic amphiphiles (lipid RPR120535 and pyrenyl polyamine), their use for the complexation of plasmid DNA, and their efficiency in transfecting cells in vitro. The experiments have shown that the efficiency of transfection is higher when using SWNT instead of MWNT, and that transfection effi ciency is similar or slightly higher when using nanoplexes (SWNT/lipid RPR120535/DNA) instead of lipoplexes (lipid RPR120535/DNA) and several orders of magnitude higher than that of naked DNA. This study therefore shows both that the transfection is better when using SWNTs and that it is dependent on the nature of the amphiphilic molecules adsorbed on the nanotubes.
Tsinghua University Press
Cyrille Richard,Nathalie Mignet,Céline Largeau,Virginie Escriou,Michel Bessodes,Daniel Scherman, Functionalization of single- and multi-walled carbon nanotubes with cationic amphiphiles for plasmid DNA complexation and transfection. NanoRes.2009, 2: 638-647