Direct imaging of titania nanotubes located in mouse neural stem cell nuclei
Titania nanotubes, mouse neural stem cells, nucleus, confocal imaging, atomic force microscopy
Titania nanotubes (TiO2-NTs) are a potential drug vehicle for use in nanomedicine. To this end, a preliminary study of the interaction of a model cell with TiO2-NTs has been carried out. TiO2-NTs were first conjugated with a fl uorescent label, fl uorescein isothiocyanate (FITC). FITC-conjugated titania nanotubes (FITC-TiO2-NTs) internalized in mouse neural stem cells (NSCs, line C17.2) can be directly imaged by confocal microscopy. The confocal imaging showed that FITC-TiO2-NTs readily entered into the cells. After co-incubation with cells for 24 h, FITC-TiO2-NTs localized around the cell nucleus without crossing the karyotheca. More interestingly, the nanotubes passed through the karyotheca entering the cell nucleus after co-incubation for 48 h. Atomic force microscopy (AFM) and transmission electron microscopy (TEM) were also employed in tracking the nanotubes in the cell. These results will be of benefi t in future studies of TiO2-NTs for use as a drug vehicle, particularly for DNA-targeting drugs.
Tsinghua University Press
Yanli Wang,Jia Wang,Xiaoyong Deng,Jiao Wang,Haifang Wang,Minghong Wu,Zheng Jiao,Yuanfang Liu, Direct imaging of titania nanotubes located in mouse neural stem cell nuclei. NanoRes.2009, 2: 543-552