Article Title

Control of aggregate size of polyethyleneimine-coated magnetic nanoparticles for magnetofection

Authors

Xiaoliang Wang, Nano Biomedicine Research Center, Med-X Research Institute, Shanghai Jiaotong University, 1954 Huashan Road, Shanghai 200030, China
Linzhu Zhou, Nano Biomedicine Research Center, Med-X Research Institute, Shanghai Jiaotong University, 1954 Huashan Road, Shanghai 200030, China
Yongjie Ma, Nano Biomedicine Research Center, Med-X Research Institute, Shanghai Jiaotong University, 1954 Huashan Road, Shanghai 200030, China
Xu Li, Nano Biomedicine Research Center, Med-X Research Institute, Shanghai Jiaotong University, 1954 Huashan Road, Shanghai 200030, China
Hongchen Gu, Nano Biomedicine Research Center, Med-X Research Institute, Shanghai Jiaotong University, 1954 Huashan Road, Shanghai 200030, China

Keywords

Magnetofection; transfection; magnetic nanoparticles, polyethyleneimine (PEI)

Abstract

Using magnetic nanoparticles to enhance gene transfection, a recently developed technique termed magnetofection, has been shown to be a powerful technology in gene delivery. The most widely used magnetic nanoparticles in this area are those coated with polyethyleneimine, which is a well known non- viral transfection agent. In this article, we report methods to control the aggregate size of polyethyleneimine- coated magnetite particles. These particles were then used to enhance transfection of green fl uorescent protein (GFP) into NIH 3T3 cells in vitro. We find that the aggregate size of the particles has a great effect on their performance in magnetofection, with less aggregated magnetic particles being more effective in enhancing the gene transfection.

Graphical Abstract

DOI

https://doi.org/10.1007/s12274-009-9035-6

Publisher

Tsinghua University Press

Recommended Citation

Xiaoliang Wang,Linzhu Zhou,Yongjie Ma,Xu Li,Hongchen Gu, Control of aggregate size of polyethyleneimine-coated magnetic nanoparticles for magnetofection. NanoRes.2009, 2: 365-372