•  
  •  
 
Nano Research

Article Title

Fluorescent silicon nanoparticles-based nanotheranostic agents for rapid diagnosis and treatment of bacteria-induced keratitis

Authors

Lu Zhang, Laboratory of Nanoscale Biochemical Analysis, Institute of Functional Nano and Soft Materials , and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215123, China
Xiaoyuan Ji, Laboratory of Nanoscale Biochemical Analysis, Institute of Functional Nano and Soft Materials , and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215123, China
Yuanyuan Su, Laboratory of Nanoscale Biochemical Analysis, Institute of Functional Nano and Soft Materials , and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215123, China
Xia Zhai, Laboratory of Nanoscale Biochemical Analysis, Institute of Functional Nano and Soft Materials , and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215123, China
Hua Xu, Department of Ophthalmology, Children’s Hospital of Soochow University, Soochow University, Suzhou 215123, China
Bin Song, Laboratory of Nanoscale Biochemical Analysis, Institute of Functional Nano and Soft Materials , and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215123, China
Airui Jiang, Laboratory of Nanoscale Biochemical Analysis, Institute of Functional Nano and Soft Materials , and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215123, China
Daoxia Guo, Laboratory of Nanoscale Biochemical Analysis, Institute of Functional Nano and Soft Materials , and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215123, China
Yao He, Laboratory of Nanoscale Biochemical Analysis, Institute of Functional Nano and Soft Materials , and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215123, China

Keywords

bacterial keratitis, bio-imaging, theranostic probe, silicon nanoparticles

Abstract

Recommended as a medical emergency, infectious keratitis with an acute and rapid disease progression can lead to serious damage of vision and even blindness. Herein, we present a kind of theranostic agents, which are made of vancomycin (Van)-modified fluorescent silicon nanoparticles (SiNPs-Van), enabling rapid and non-invasive diagnosis and treatment of Gram-positive bacteria-induced keratitis in a simultaneous manner. Typically, the resultant SiNPs-Van nanoagents have an ability of imaging bacteria in a short time both in vitro (5 min) and in vivo (10 min), making them an efficacious diagnostic agent for the detection of bacterial keratitis. In addition, the SiNPs-Van feature distinct antimicrobial activity, with superior activity of 92.5% at a concentration of 0.5 μg/mL against Staphylococcus aureus (S. aureus); comparatively, the antimicrobial rate of free vancomycin is 23.3% at the same concentration. We further explore the SiNPs-Van agents as eye drops for therapy of S. aureus-induced bacterial keratitis on rat model. Represented by slit-lamp scores, the keratitis severity of SiNPs-Van-treated corneas is 3.4, which is 59.6% and 77.3% slighter than vancomycin-(8.2 score) and PBS-treated corneas (15.0 score), respectively. The infected corneas recover to normal (1 score) after 7-d of SiNPs-Van treatment. Above results suggest that the SiNPs-Van could serve as a new kind of high-quality nanotheranostic agents, especially suitable for simultaneous diagnosis and therapy of Gram-positive bacteria keratitis.

Publisher

Tsinghua University Press

Share

COinS