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Nano Research

Article Title

Efficient elimination of multidrug-resistant bacteria using copper sulfide nanozymes anchored to graphene oxide nanosheets

Authors

Wanshun Wang, Guangdong Key Lab of Orthopedic Technology and Implant Materials, General Hospital of Southern Theater Command of PLA, The Second Clinical Medical College and Department of Graduate School of Guangzhou University of Chinese Medicine, The Second School of Clinical Medicine of Southern Medical University, Guangzhou 510010, China
Binglin Li, Guangdong Key Lab of Orthopedic Technology and Implant Materials, General Hospital of Southern Theater Command of PLA, The Second Clinical Medical College and Department of Graduate School of Guangzhou University of Chinese Medicine, The Second School of Clinical Medicine of Southern Medical University, Guangzhou 510010, China
Huili Yang, Guangdong Key Lab of Orthopedic Technology and Implant Materials, General Hospital of Southern Theater Command of PLA, The Second Clinical Medical College and Department of Graduate School of Guangzhou University of Chinese Medicine, The Second School of Clinical Medicine of Southern Medical University, Guangzhou 510010, China
Zefeng Lin, Guangdong Key Lab of Orthopedic Technology and Implant Materials, General Hospital of Southern Theater Command of PLA, The Second Clinical Medical College and Department of Graduate School of Guangzhou University of Chinese Medicine, The Second School of Clinical Medicine of Southern Medical University, Guangzhou 510010, China
Lingling Chen, Guangdong Key Lab of Orthopedic Technology and Implant Materials, General Hospital of Southern Theater Command of PLA, The Second Clinical Medical College and Department of Graduate School of Guangzhou University of Chinese Medicine, The Second School of Clinical Medicine of Southern Medical University, Guangzhou 510010, China
Zhan Li, Guangdong Key Lab of Orthopedic Technology and Implant Materials, General Hospital of Southern Theater Command of PLA, The Second Clinical Medical College and Department of Graduate School of Guangzhou University of Chinese Medicine, The Second School of Clinical Medicine of Southern Medical University, Guangzhou 510010, China
Jiayuan Ge, Guangdong Key Lab of Orthopedic Technology and Implant Materials, General Hospital of Southern Theater Command of PLA, The Second Clinical Medical College and Department of Graduate School of Guangzhou University of Chinese Medicine, The Second School of Clinical Medicine of Southern Medical University, Guangzhou 510010, China
Tao Zhang, Guangdong Key Lab of Orthopedic Technology and Implant Materials, General Hospital of Southern Theater Command of PLA, The Second Clinical Medical College and Department of Graduate School of Guangzhou University of Chinese Medicine, The Second School of Clinical Medicine of Southern Medical University, Guangzhou 510010, China
Hong Xia, Guangdong Key Lab of Orthopedic Technology and Implant Materials, General Hospital of Southern Theater Command of PLA, The Second Clinical Medical College and Department of Graduate School of Guangzhou University of Chinese Medicine, The Second School of Clinical Medicine of Southern Medical University, Guangzhou 510010, China
Lihua Li, State Key Laboratory of Luminescent Materials and Devices, Guangdong Provincial Key Laboratory of Fiber Laser Materials, School of Materials Science and Engineering, School of Physics, South China University of Technology, Guangzhou 510640, China
Yao Lu, Guangdong Key Lab of Orthopedic Technology and Implant Materials, General Hospital of Southern Theater Command of PLA, The Second Clinical Medical College and Department of Graduate School of Guangzhou University of Chinese Medicine, The Second School of Clinical Medicine of Southern Medical University, Guangzhou 510010, China Orthopedic Centre, Clinical Research Centre, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China

Keywords

antibacterial nanomaterials, nanozyme, multidrug-resistant bacteria, wound healing

Abstract

Antibacterial nanomaterials have attracted growing interest for bacterial infection therapy. However, most nanomaterials eliminate bacteria either physically or chemically, which hampers their efficacy when dealing with multidrug-resistant bacteria. To overcome this, we integrated copper sulfide (CuS) nanoparticles with active graphene oxide nanosheets (GO NSs) to synthesize a superior nanocomposite (CuS/GO NC) that acts both physically and chemically on the bacteria. CuS/GO NC was produced using a facile hydrothermal method, whereby the CuS nanoparticles grew and were uniformly dispersed on the GO NSs in situ. We found that the CuS/GO NC possesses a unique needle-like morphology that physically damages the bacterial cell membrane. CuS/GO NC also exhibits high oxidase- and peroxidase-like activity, ensuring efficient generation of the reactive oxygen species •OH from H2O2, which kills bacteria chemically. These features endow the CuS/GO NC with excellent antibacterial capabilities to kill multidrugresistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA) with only a single dose. Additionally, it was found that the CuS/GO NC accelerated the healing of infected wounds in vivo owing to its good biocompatibility as well as facilitation of cell migration and collagen secretion. This study provides a new strategy to combine the physical and chemical antibacterial modes of nanomaterials to develop more effective therapies to combat multidrug-resistant bacterial infections.

Graphical Abstract

Publisher

Tsinghua University Press

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