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Nano Research

Article Title

Nanoliposome-encapsulated caged-GABA for modulating neural electrophysiological activity with simultaneous detection by microelectrode arrays

Authors

Jingyu Xie, State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China University of Chinese Academy of Sciences, Beijing 100049, China
Yilin Song, State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China University of Chinese Academy of Sciences, Beijing 100049, China
Yuchuan Dai, State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China University of Chinese Academy of Sciences, Beijing 100049, China
Ziyue Li, State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China University of Chinese Academy of Sciences, Beijing 100049, China
Fei Gao, State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China University of Chinese Academy of Sciences, Beijing 100049, China
Xuanyu Li, University of Chinese Academy of Sciences, Beijing 100049, China Beijing Engineering Research Center for BioNanotechnology, CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for NanoScience and Technology, Beijing 100190, China
Guihua Xiao, State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China University of Chinese Academy of Sciences, Beijing 100049, China
Yu Zhang, State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China University of Chinese Academy of Sciences, Beijing 100049, China
Hao Wang, State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China University of Chinese Academy of Sciences, Beijing 100049, China
Zeying Lu, State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China University of Chinese Academy of Sciences, Beijing 100049, China
Xingyu Jiang, Beijing Engineering Research Center for BioNanotechnology, CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for NanoScience and Technology, Beijing 100190, China Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen 518055, China
Wenfu Zheng, Beijing Engineering Research Center for BioNanotechnology, CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for NanoScience and Technology, Beijing 100190, China
Xinxia Cai, State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China University of Chinese Academy of Sciences, Beijing 100049, China

Keywords

neural modulation, nanoliposome, microelectrode array, light-sensitive drug

Abstract

Effective and precise neural modulation with real-time detection in the brain is of great importance and represents a significant challenge. Nanoliposome-encapsulated light-sensitive compounds have excellent characteristics such as high temporal and spatial resolution, delayed drug clearance, and restricted drug biodistribution for neural modulation. In this study, we developed a nanoliposome-based delivery system for ruthenium-based caged GABA compounds (Nanolipo-Ru) to modulate neural activity and allow for real-time monitoring using the microelectrode arrays (MEAs). The Nanolipo-Ru nanoparticles had an average size of 134.10 ± 4.30 nm and exhibited excellent stability for seven weeks. For the in vivo experiment in the rat, release of GABA by Nanolipo-Ru under blue light illumination resulted in an average firing rate reduction in interneurons and pyramidal neurons in the same brain region of 79.4% and 81.6%, respectively. Simultaneously, the average power of local field potentials in the 0–15 Hz range degraded from 4.34 to 0.85 mW. In addition, the Nanolipo-Ru nanoparticles have the potential to provide more flexible timing of modulation than unencapsulated RuBi-GABA in the experiments. These results indicated that Nanolipo-Ru could be an effective platform for regulating neuronal electrophysiology. Furthermore, nanoliposomes with appropriate modifications would render promising utilities for targeting of specific types of neurons in the future.

Graphical Abstract

Publisher

Tsinghua University Press

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