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Nano Research

Article Title

Nanoparticle-immersed paper imprinting mass spectrometry imaging reveals uptake and translocation mechanism of pesticides in plants

Authors

Xinzhou Wu, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou 510642, China Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of Education, South China Agricultural University, Guangzhou 510642, China
Run Qin, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou 510642, China Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of Education, South China Agricultural University, Guangzhou 510642, China
Hanxiang Wu, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou 510642, China Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of Education, South China Agricultural University, Guangzhou 510642, China
Guangkai Yao, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou 510642, China Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of Education, South China Agricultural University, Guangzhou 510642, China
Yue Zhang, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou 510642, China Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of Education, South China Agricultural University, Guangzhou 510642, China
Ping Li, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou 510642, China Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of Education, South China Agricultural University, Guangzhou 510642, China
Yizhu Xu, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou 510642, China Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of Education, South China Agricultural University, Guangzhou 510642, China
Zhixiang Zhang, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou 510642, China Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of Education, South China Agricultural University, Guangzhou 510642, China
Zhibin Yin, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China
Hanhong Xu, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou 510642, China Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of Education, South China Agricultural University, Guangzhou 510642, China

Keywords

gold nanoparticles, nanoparticle-immersed paper imprinting, mass spectrometry imaging, plant translocation mechanism, carrier-mediated pesticides

Abstract

Design and discovery of carrier-mediated modified pesticides are vital for reducing pesticide dosage and increasing utilization, yet it remains a great challenge due to limited insights into plant translocation mechanisms. Nanostructure/nanoparticle assisted laser desorption/ionization strategy has established itself as a preferential analytical tool for biological tissue analysis, whereas potential applications in plant sciences are hindered with regard to the inability to slice plant leaves and petals. Herein, we report gold nanoparticle (AuNP)-immersed paper imprinting mass spectrometry imaging (MSI) for the spatiotemporal visualization of pesticide translocation in plant leaves. This approach plays a dual role in preserving spatial information and improving ionization efficiency for pesticides regardless of imaging artifacts due to homogenous AuNP deposition. Using this MSI platform, we proposed the elaborate plant translocation mechanism of agrochemicals for the first time, which is currently poorly understood. The dynamic processes of carrier-mediated pesticides can be clearly visualized, including crossing of plasma membranes by transporters, translocation downward in stems through the phloem, diffusion to the xylem and, conversely, accumulation at margins of the treated leaves. Moreover, this AuNP-assisted paper imprinting method could be highly compatible with laser-based MSI instruments, expediting researches across a broad range of fields, especially in nanomaterial development and life sciences.

Graphical Abstract

Publisher

Tsinghua University Press

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