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Nano Research

Article Title

A neurotrophic peptide-functionalized self-assembling peptide nanofiber hydrogel enhances rat sciatic nerve regeneration

Authors

Jiaju Lu, State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China
Xun Sun, Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China School of Medicine, Nankai University, Tianjin 300071, China
Heyong Yin, Experimental Surgery and Regenerative Medicine, Department of Surgery, Ludwig-Maximilians-University, Munich 80336, Germany
Xuezhen Shen, Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China School of Medicine, Nankai University, Tianjin 300071, China
Shuhui Yang, State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China
Yu Wang, Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China
Wenli Jiang, Department of Ultrasound, Beijing Hospital, Beijing 100730, China
Yue Sun, Center of Biomedical Analysis, Tsinghua University, Beijing 100084, China
Lingyun Zhao, State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China
Xiaodan Sun, State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China
Shibi Lu, Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China
Antonios G. Mikos, Department of Bioengineering, Bioscience Research Collaborative, Rice University, Texas 77030, USA
Jiang Peng, Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China Co-innovation Center of Neuroregeneration, Nantong University, Nantong 226007, China
Xiumei Wang, State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China

Keywords

peripheral nerve regeneration, self-assembling peptide, hydrogel, neurotrophic peptide, intraluminal microenvironment

Abstract

ABSTRACT Nerve guidance conduit (NGC) is a potential alternative to autologous nerve for peripheral nerve regeneration. A promising therapeutic strategy is to modify the nerve guidance conduit intraluminal microenvironment using physical and/or chemical guidance cues. In this study, a neurotrophic peptide-functionalized self-assembling peptide nanofiber hydrogel that could promote PC12 cell adhesion, proliferation, and neuronal differentiation in vitro was prefilled in the lumen of a hollow chitosan tube (hCST) to accelerate axonal regeneration in a rat sciatic nerve defect model. The functionalized self-assembling peptide was developed by introducing a neurotrophic peptide (RGI, RGIDKRHWNSQ) derived from brain-derived neurotrophic factor (BDNF) to the C-terminus of the self-assembling peptide RADA16-I (Ac-(RADA)4-CONH2). Morphological, histological, electrophysiological, and functional analyses demonstrated that the RGI-functionalized, self-assembling, peptide nanofiber hydrogel RAD/RGI could produce a neurotrophic microenvironment that markedly improved axonal regeneration with enhanced re-myelination and motor functional recovery.

Graphical Abstract

Publisher

Tsinghua University Press

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