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Nano Research

Article Title

Enzyme@silica nanoflower@metal-organic framework hybrids: A novel type of integrated nanobiocatalysts with improved stability

Authors

Yingjie Du, School of Chemical Engineering and Technology, Hebei University of Technology, 8 Guangrong Road, Hongqiao District, Tianjin 300130, China
Jing Gao, School of Chemical Engineering and Technology, Hebei University of Technology, 8 Guangrong Road, Hongqiao District, Tianjin 300130, China Hebei Provincial Key Lab of Green Chemical Technology and High Efficient Energy Saving, Hebei University of Technology, 8 Guangrong Road, Hongqiao District, Tianjin 300130, China
Huajiao Liu, School of Chemical Engineering and Technology, Hebei University of Technology, 8 Guangrong Road, Hongqiao District, Tianjin 300130, China
Liya Zhou, School of Chemical Engineering and Technology, Hebei University of Technology, 8 Guangrong Road, Hongqiao District, Tianjin 300130, China
Li Ma, School of Chemical Engineering and Technology, Hebei University of Technology, 8 Guangrong Road, Hongqiao District, Tianjin 300130, China
Ying He, School of Chemical Engineering and Technology, Hebei University of Technology, 8 Guangrong Road, Hongqiao District, Tianjin 300130, China
Zhihong Huang, School of Chemical Engineering and Technology, Hebei University of Technology, 8 Guangrong Road, Hongqiao District, Tianjin 300130, China
Yanjun Jiang, School of Chemical Engineering and Technology, Hebei University of Technology, 8 Guangrong Road, Hongqiao District, Tianjin 300130, China Hebei Provincial Key Lab of Green Chemical Technology and High Efficient Energy Saving, Hebei University of Technology, 8 Guangrong Road, Hongqiao District, Tianjin 300130, China

Keywords

silica nanoflower, metal-organic framework, hybrids, nanobiocatalyst, immobilization

Abstract

ABSTRACT A novel integrated nanobiocatalyst system based on an enzyme@silica nanoflower@metal-organic framework (enzyme@SNF@ZIF-8) structure with improved stability is fabricated for the first time. The versatility of this system is validated using penicillin G acylase (PGA) and catalase (CAT) as model enzymes. The microporous ZIF-8 layer can be controlled by varying the number of ZIF-8 coating cycles, which produces PGA@SNF@ZIF-8 nanobiocatalysts with different ZIF-8 layer thicknesses. After the second ZIF-8 coating cycle, a PGA@SNF@ZIF-8(2) structure with a homogeneous and well-intergrown ZIF-8 layer is formed, which possesses excellent mechanical and chemical stability. Moreover, PGA@SNF@ZIF-8(2) shows improved thermal/storage stability and reusability compared with free PGA and PGA immobilized on silica nanoflowers (PGA@SNF). The obtained CAT-based nanobiocatalysts (CAT@SNF@ZIF-8(2)) also show excellent catalytic performance.

Graphical Abstract

Publisher

Tsinghua University Press

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