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Nano Research

Article Title

Promoting osteogenic differentiation in pre-osteoblasts and reducing tibial fracture healing time using functional nanofibers

Authors

Gu Cheng, The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology & Department of Oral and Maxillofacial Trauma and Plastic Surgery, Wuhan University Stomatological Hospital, Wuhan University, Wuhan 430079, China
Jiajia Chen, Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, Hubei Key Lab of Biomass Resource Chemistry and Environmental Biotechnology, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China
Qun Wang, Department of Chemical and Biological Engineering, Iowa State University, Ames, IA 50014, USA
Xuewen Yang, The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology & Department of Oral and Maxillofacial Trauma and Plastic Surgery, Wuhan University Stomatological Hospital, Wuhan University, Wuhan 430079, China
Yuet Cheng, The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology & Department of Oral and Maxillofacial Trauma and Plastic Surgery, Wuhan University Stomatological Hospital, Wuhan University, Wuhan 430079, China
Zhi Li, The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology & Department of Oral and Maxillofacial Trauma and Plastic Surgery, Wuhan University Stomatological Hospital, Wuhan University, Wuhan 430079, China
Hu Tu, Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, Hubei Key Lab of Biomass Resource Chemistry and Environmental Biotechnology, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China
Hongbing Deng, Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, Hubei Key Lab of Biomass Resource Chemistry and Environmental Biotechnology, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China
Zubing Li, The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology & Department of Oral and Maxillofacial Trauma and Plastic Surgery, Wuhan University Stomatological Hospital, Wuhan University, Wuhan 430079, China

Keywords

silk fibroin, polycaprolactone, scaffold, bone tissue engineering

Abstract

ABSTRACT Various composite materials are now used as artificial tissue substitutes, and are defining new frontiers in tissue engineering. In the present study, composite membranes based on silk fibroin (SF) were fabricated to form a synthetic periosteum. The fabricated membranes were physicochemically characterized by their morphology, porosity, biocompatibility, biodegradability, chemical structure, and mechanical properties. Following the addition of polycaprolactone (PCL) to the silk fibers, there was a 3–5-fold increase in the elongation at break compared with the pure silk membranes, and surface wettability was retained. The degradation time of the SF within the membranes was also prolonged by adding PCL. Compared with pure PCL membranes or plastic culture plates, the SF-based membranes significantly enhanced the cellular viability and osteogenic differentiation capability of MC3T3-E1 cells. Higher expression levels of osteogenic differentiation markers (runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), and osteopontin (OP)) further supported the use of the SF component in bone-related applications. A non-rigid internal fixation (non-RIF) fracture model that healed via endochondral bone formation was created, and fracture callus samples were collected to perform micro-computed tomography, histology, and immunohistochemistry analyses at 8 weeks after surgery. A smaller bone volume accompanied by a mineralized bony callus was observed in SF/PCL membrane-treated rats. Immunohistochemistry also indicated that the SF/PCL membrane-treated rats exhibited increased osteocalcin expression but reduced collagen type X expression. These findings could lead to an alternative strategy for treating comminuted fractures with enhanced intramembranous ossification and reduced endochondral ossification.

Graphical Abstract

Publisher

Tsinghua University Press

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