Inhibition of osteosarcoma growth and metastasis using a polysaccharide derivative of Amy-g-PLLD for the delivery of AEG-1 siRNA
osteosarcoma, amylose, gene delivery, astrocyte elevated gene-1 (AEG-1) small-interfering RNA (siRNA)
ABSTRACT Osteosarcoma is the most common primary malignant neoplasm of the bone in children and adolescents and has a high risk of relapse and metastasis. Of the various methods to treat osteosarcoma, the use of genetic approaches to inhibit the rapid growth of osteosarcoma while limiting tumor metastasis has presented a challenge in its implementation. Here, we successfully synthesized a polysaccharide derivative (Amy-g-PLLD) for delivery of astrocyte elevated gene-1 (AEG-1) small-interfering RNA (siRNA) (siAEG-1), and used it for the first time to suppress osteosarcoma tumors in vitro and in vivo. Amy-g-PLLD/ siAEG-1 complexes were delivered into 143B human osteosarcoma cells with low resultant cytotoxicity. Osteosarcoma tumor proliferation and invasion were inhibited in vitro. Intratumoral injection of Amy-g-PLLD/siAEG-1 complexes markedly inhibited tumor growth and lung metastasis in 143B tumor-bearing mice. This biocompatible and effective approach employing a natural materialsiRNA complex should pave the way for more genetic research in treating osteosarcoma.
Tsinghua University Press
Fen Wang,Jiadong Pang,Leilei Huang,Ran Wang,Qing Jiang,Liming Zhang,Kang Sun, Inhibition of osteosarcoma growth and metastasis using a polysaccharide derivative of Amy-g-PLLD for the delivery of AEG-1 siRNA. NanoRes.2018, 11(7): 3886–3898