A strategy for accurate detection of glucose in human serum and whole blood based on an upconversion nanoparticles-polydopamine nanosystem
upconversion nanoparticles, polydopamine, Förster resonance energy transfer, blood glucose, bioassay
ABSTRACT The accurate detection of blood glucose is of critical importance in the diagnosis and management of diabetes and its complications. Herein, we report a novel strategy based on an upconversion nanoparticles-polydopamine (UCNPs-PDA) nanosystem for the accurate detection of glucose in human serum and whole blood through a simple blending of test samples with ligand-free UCNPs, dopamine, and glucose oxidase (GOx). Owing to the high affinity of lanthanide ions exposed on the surface of ligand-free UCNPs, dopamine monomers could spontaneously attach to the UCNPs and further polymerize to form a PDA shell, resulting in a remarkable upconversion luminescence (UCL) quenching (97.4%) of UCNPs under 980-nm excitation. Such UCL quenching can be effectively inhibited by H2O2 produced from the GOx/glucose enzymatic reaction, thus enabling the detection of H2O2 or glucose based on the UCL quenching/inhibition bioassay. Owing to the highly sensitive UCL response and background-free interference of the UCNPs-PDA nanosystem, we achieved a sensitive, selective, and high-throughput bioassay for glucose in human serum and whole blood, thereby revealing the great potential of the UCNPs-PDA nanosystem for the accurate detection of blood glucose or other H2O2-generated biomolecules in clinical bioassays.
Tsinghua University Press
Yan Liu,Datao Tu,Wei Zheng,Lianyu Lu,Wenwu You,Shanyong Zhou,Ping Huang,Renfu Li,Xueyuan Chen, A strategy for accurate detection of glucose in human serum and whole blood based on an upconversion nanoparticles-polydopamine nanosystem. NanoRes.2018, 11(6): 3164–3174