•  
  •  
 
Nano Research

Article Title

Nontoxic engineered virus nanofibers as an efficient agent for the prevention and detection of fungal infection

Authors

Yicun Wang, Jilin Provincial Key Laboratory on Molecular and Chemical Genetic, The Second Hospital of Jilin University, Changchun 130024, China
Hongxi Shi, Key Laboratory of Molecular Epigenetics of MOE and Institute of Genetics & Cytology, Northeast Normal University, Changchun 130024, China
Shuai Dong, Key Laboratory of Molecular Epigenetics of MOE and Institute of Genetics & Cytology, Northeast Normal University, Changchun 130024, China
Yan Li, Key Laboratory of Molecular Epigenetics of MOE and Institute of Genetics & Cytology, Northeast Normal University, Changchun 130024, China
Meng Wang, Department of Respiratory Medicine, Harbin Medical University Cancer Hospital, Harbin 150081, China
Yanyan Huai, Key Laboratory of Molecular Epigenetics of MOE and Institute of Genetics & Cytology, Northeast Normal University, Changchun 130024, China
Xintong Zhang, Key Laboratory of Molecular Epigenetics of MOE and Institute of Genetics & Cytology, Northeast Normal University, Changchun 130024, China
Xi Chen, Key Laboratory of Molecular Epigenetics of MOE and Institute of Genetics & Cytology, Northeast Normal University, Changchun 130024, China
Chuanbin Mao, School of Materials Science and Engineering, Zhejiang University, Hangzhou 310027, China Department of Chemistry & Biochemistry, Stephenson Life Science Research Center, University of Oklahoma, Norman 73019-5300, USA
Xiang Gao, Key Laboratory of Molecular Epigenetics of MOE and Institute of Genetics & Cytology, Northeast Normal University, Changchun 130024, China
Li Wang, Key Laboratory of Molecular Epigenetics of MOE and Institute of Genetics & Cytology, Northeast Normal University, Changchun 130024, China

Keywords

phage, nanofiber, Candida albicans, diagnosis, vaccine

Abstract

ABSTRACT Candida albicans (C. albicans) infection has a high mortality rate in immunocompromised patients. Owing to the inefficiency of the current diagnostic system and the absence of licensed vaccines against candidiasis, the prevention of C. albicans infection remains a challenge. C. albicans infection can be evaluated and prevented by the anti-secreted aspartyl proteinase 2 antibody (anti-Sap2 IgG) and Hsp90 antibody (anti-Hsp90 IgG). In this study, to explore a new agent for the improvement of the diagnosis and the prevention of C. albicans infection, an engineered fd bacteriophage, which is considered a human-safe virus nanofiber, was designed and prepared with two epitopes that could induce and capture anti-Sap2 IgG and anti-Hsp90 IgG. The dual-display phage was employed as a novel capture probe to develop a new enzyme-linked immunosorbent assay (ELISA) method, which significantly improved the detection rate compared with those of the ELISA in which recombinant protein Sap2 was used as coating antigen to capture the specific antibodies (rSap2-ELISA) and the ELISA in which recombinant protein Hsp90 was used as coating antigen to capture the specific antibodies (rHsp90-ELISA). In addition, the nanofibers acted as a potential vaccine to immunize mice, as well as recombinant proteins, more efficiently mediated humoral and cellular immune responses, decreased levels of C. albicans colonization, and increased the survival rates in C. albicans-infected mice. Therefore, the phage dual-display nanofiber has been shown to be a powerful bifunctional agent for protection against and sensitive detection of clinical infections, which has the potential to be widely used in the life sciences, clinical medicine, and environmental sciences.

Graphical Abstract

Publisher

Tsinghua University Press

Share

COinS