•  
  •  
 
Nano Research

Article Title

Gold nanoshells: Contrast agents for cell imaging by cardiovascular optical coherence tomography

Authors

Jie Hu, Fluorescence Imaging Group, Departamento de Física de Materiales, Instituto Nicolás Cabrera, Facultad de Ciencias, Universidad Autónoma de Madrid, Madrid 28049, Spain
Francisco Sanz-Rodríguez, Fluorescence Imaging Group, Departamento de Física de Materiales, Instituto Nicolás Cabrera, Facultad de Ciencias, Universidad Autónoma de Madrid, Madrid 28049, Spain Instituto Ramón y Cajal de Investigación Sanitaria, Hospital Ramón y Cajal, Madrid 28034, Spain Departamento de Biología, Universidad Autónoma de Madrid, Madrid 28049, Spain
Fernando Rivero, Cardiology Department, Hospital Universitario de la Princesa, IIS-IP, Universidad Autónoma de Madrid, Madrid 28006, Spain
Emma Martín Rodríguez, Fluorescence Imaging Group, Departamento de Física de Materiales, Instituto Nicolás Cabrera, Facultad de Ciencias, Universidad Autónoma de Madrid, Madrid 28049, Spain Instituto Ramón y Cajal de Investigación Sanitaria, Hospital Ramón y Cajal, Madrid 28034, Spain
Río Aguilar Torres, Cardiology Department, Hospital Universitario de la Princesa, IIS-IP, Universidad Autónoma de Madrid, Madrid 28006, Spain
Dirk H. Ortgies, Fluorescence Imaging Group, Departamento de Física de Materiales, Instituto Nicolás Cabrera, Facultad de Ciencias, Universidad Autónoma de Madrid, Madrid 28049, Spain Instituto Ramón y Cajal de Investigación Sanitaria, Hospital Ramón y Cajal, Madrid 28034, Spain
José García Solé, Fluorescence Imaging Group, Departamento de Física de Materiales, Instituto Nicolás Cabrera, Facultad de Ciencias, Universidad Autónoma de Madrid, Madrid 28049, Spain
Fernando Alfonso, Cardiology Department, Hospital Universitario de la Princesa, IIS-IP, Universidad Autónoma de Madrid, Madrid 28006, Spain
Daniel Jaque, Fluorescence Imaging Group, Departamento de Física de Materiales, Instituto Nicolás Cabrera, Facultad de Ciencias, Universidad Autónoma de Madrid, Madrid 28049, Spain Instituto Ramón y Cajal de Investigación Sanitaria, Hospital Ramón y Cajal, Madrid 28034, Spain

Keywords

OCT intravascular imaging, gold nanoparticles, 3D imaging

Abstract

ABSTRACT Optical coherence tomography (OCT) has gained considerable attention in interventional cardiovascular medicine and is currently used in clinical settings to assess atherosclerotic lesions and to optimize stent placement. Artery imaging at the cellular level constitutes the first step towards cardiovascular molecular imaging, which represents a major advance in the development of personalized noninvasive therapies. In this work, we demonstrate that cardiovascular OCT can be used to detect individual cells suspended in biocompatible fluids. Importantly, the combination of this catheter-based clinical technique with gold nanoshells (GNSs) as intracellular contrast agents led to a substantial enhancement in the backscattered signal produced by individual cells. This cellular contrast enhancement was attributed to the large backscattering cross-section of GNSs at the OCT laser wavelength (1,300 nm). A simple intensity analysis of OCT cross-sectional images of suspended cells makes it possible to identify the sub-population of living cells that successfully incorporated GNSs. The generalizability of this method was demonstrated using two different cell lines (HeLa and Jurkat cells). This work provides novel insights into cardiovascular molecular imaging using specifically modified GNSs.

Graphical Abstract

Publisher

Tsinghua University Press

Share

COinS