Co-delivery of mRNA and SPIONs through amino-ester nanomaterials
amino-ester nanomaterials, lipid-like nanoparticles (LLNs), dual-functional, superparamagnetic iron oxide nanoparticles (SPIONs), mRNA delivery, magnetic resonance imaging (MRI)
ABSTRACT Nanoparticles have been widely explored for combined therapeutic and diagnostic applications. For example, lipid-based nanoparticles have been used to encapsulate multiple types of agents and achieve multi-functions. Herein, we enabled a co-delivery of mRNA molecules and superparamagnetic iron oxide nanoparticles (SPIONs) by using an amino-ester lipid-like nanomaterial. An orthogonal experimental design was used to identify the optimal formulation. The optimal formulation, MPA-Ab-8 LLNs, not only showed high encapsulation of both mRNA and SPIONs, but also increased the r2 relaxivity of SPIONs by more than 1.5-fold in vitro. MPA-Ab-8 LLNs effectively delivered mRNA and SPIONs into cells, and consequently induced high protein expression as well as strong MRI contrast. Consistent herewith, we observed both mRNA-mediated protein expression and an evident negative contrast enhancement of MRI signal in mice. In conclusion, amino-ester nanomaterials demonstrate great potential as delivery vehicles for theranostic applications.
Tsinghua University Press
Xiao Luo,Weiyu Zhao,Bin Li,Xinfu Zhang,Chengxiang Zhang,Anna Bratasz,Binbin Deng,David W. McComb,Yizhou Dong, Co-delivery of mRNA and SPIONs through amino-ester nanomaterials. NanoRes.2018, 11(10): 5596–5603