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Nano Research

Article Title

An intelligent near-infrared light activatable nanosystem for accurate regulation of zinc signaling in living cells

Authors

Wei Li, Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun 130022, China University of Chinese Academy of Sciences, Beijing 100039, China
Zhen Liu, Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun 130022, China
Zhaowei Chen, Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun 130022, China University of Chinese Academy of Sciences, Beijing 100039, China
Lihua Kang, Stem Cell and Cancer Center, First Affiliated Hospital, Jilin University, Changchun 130061, China
Yijia Guan, Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun 130022, China University of Chinese Academy of Sciences, Beijing 100039, China
Jinsong Ren, Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun 130022, China
Xiaogang Qu, Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun 130022, China

Keywords

nanocarrier, zinc, upconversion, spiropyran, cellular manipulation

Abstract

ABSTRACT Accurate regulation of cellular zinc signaling is imperative to decipher underlying zinc functions and develop new therapeutic agents. However, the ability to modulate zinc in a spatiotemporal manner remains elusive. We herein report an intelligent spiropyran-upconversion (SP-UCNPs) based nanosystem that enables near-infrared (NIR) light-controlled zinc release at precise times and locations. The magnitude of zinc release can be simply manipulated by varying the duration of NIR irradiation. Moreover, the utilization of NIR light not only showed little damage to cells but also significantly improved penetration depth. By evaluating activity of a model protein, phosphatase 2A, we further validated zinc signaling activation. Importantly, our strategy may be broadly applicable to other types of metal ions, like the ubiquitous second messenger calcium. More importantly, our strategy can potentially enable the precise control of specific signaling pathways of metal ions while minimizing cellular damage, facilitating the advanced manipulation of cellular dynamics.

Graphical Abstract

Publisher

Tsinghua University Press

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