Article Title
Tailoring RGD local surface density at the nanoscale toward adult stem cell chondrogenic commitment
Authors
Anna Lagunas, Networking Biomedical Research Center (CIBER), C/Monforte de Lemos 3-5, Pabellón 11 Planta 0, Madrid 28029, Spain
Institute for Bioengineering of Catalonia (IBEC), Baldiri-Reixac 10-12, Barcelona 08028, Spain
Iro Tsintzou, Institute for Bioengineering of Catalonia (IBEC), Baldiri-Reixac 10-12, Barcelona 08028, Spain
Yolanda Vida, Instituto de Investigación Biomédica de Málaga (IBIMA), Department of Organic Chemistry, Universidad de Málaga (UMA), Málaga 29071, Spain
Andalusian Centre for Nanomedicine and Biotechnology-BIONAND, Parque Tecnológico de Andalucía, Málaga 29590, Spain
Daniel Collado, Instituto de Investigación Biomédica de Málaga (IBIMA), Department of Organic Chemistry, Universidad de Málaga (UMA), Málaga 29071, Spain
Andalusian Centre for Nanomedicine and Biotechnology-BIONAND, Parque Tecnológico de Andalucía, Málaga 29590, Spain
Ezequiel Pérez-Inestrosa, Instituto de Investigación Biomédica de Málaga (IBIMA), Department of Organic Chemistry, Universidad de Málaga (UMA), Málaga 29071, Spain
Andalusian Centre for Nanomedicine and Biotechnology-BIONAND, Parque Tecnológico de Andalucía, Málaga 29590, Spain
Cristina Rodríguez Pereira, Unidad de Bioingeniería Tisular y Terapia Celular (GBTTC-CHUAC), Grupo de Reumatología, Instituto de Investigación Biomédica de A Corua (INIBIC), Complexo Hospitalario Universitario de A Corua (CHUAC), Sergas, Universidade da Corua (UDC), As Xubias, 84, Corua 15006 A, Spain
Joana Magalhaes, Networking Biomedical Research Center (CIBER), C/Monforte de Lemos 3-5, Pabellón 11 Planta 0, Madrid 28029, Spain
Unidad de Bioingeniería Tisular y Terapia Celular (GBTTC-CHUAC), Grupo de Reumatología, Instituto de Investigación Biomédica de A Corua (INIBIC), Complexo Hospitalario Universitario de A Corua (CHUAC), Sergas, Universidade da Corua (UDC), As Xubias, 84, Corua 15006 A, Spain
José A. Andrades, Cell Biology, Genetics and Physiology Department, University de Málaga (UMA), Campus Teatinos, Málaga 29071, Spain
Networking Biomedical Research Center (CIBER), C/Monforte de Lemos 3-5, Pabellón 11 Planta 0, Madrid 28029, Spain
Josep Samitier, Institute for Bioengineering of Catalonia (IBEC), Baldiri-Reixac 10-12, Barcelona 08028, Spain
Networking Biomedical Research Center (CIBER), C/Monforte de Lemos 3-5, Pabellón 11 Planta 0, Madrid 28029, Spain
Department of Engineering Electronics, University of Barcelona (UB), Martí i Franquès 1-11, Barcelona 08028, Spain
Keywords
dendrimer, arginine-glycine-aspartic acid (RGD), nanopattern, human mesenchymal stem cells (hMSCs), chondrogenesis
Abstract
ABSTRACT Arginine-glycine-aspartic acid (RGD) dendrimer-based nanopatterns on poly(Llactic acid) were used as bioactive substrates to evaluate the impact of the RGD local surface density on the chondrogenic induction of adult human mesenchymal stem cells. During chondrogenic commitment, active extracellular matrix (ECM) remodeling takes place, playing an instructive role in the differentiation process. Although three-dimensional environments such as pellet or micromass cultures are commonly used for in vitro chondrogenic differentiation, these cultures are rather limited with respect to their ability to interrogate cells in cell–ECM interactions. In the present study, the nanopatterns of the tunable RGD surface density were obtained as a function of the initial dendrimer concentration. The local RGD surface density was quantified through probability contour plots for the minimum interparticle distance, constructed from the corresponding atomic force microscopy images, and correlated with the cell adhesion and differentiation response. The results revealed that the local RGD surface density at the nanoscale acts as a regulator of chondrogenic commitment, and that intermediate adhesiveness of cells to the substrates favors mesenchymal cell condensation and early chondrogenic differentiation.
Graphical Abstract

Publisher
Tsinghua University Press
Recommended Citation
Anna Lagunas,Iro Tsintzou,Yolanda Vida,Daniel Collado,Ezequiel Pérez-Inestrosa,Cristina Rodríguez Pereira,Joana Magalhaes,José A. Andrades,Josep Samitier, Tailoring RGD local surface density at the nanoscale toward adult stem cell chondrogenic commitment. NanoRes.2017, 10(6): 1959–1971