Tailoring RGD local surface density at the nanoscale toward adult stem cell chondrogenic commitment
dendrimer, arginine-glycine-aspartic acid (RGD), nanopattern, human mesenchymal stem cells (hMSCs), chondrogenesis
ABSTRACT Arginine-glycine-aspartic acid (RGD) dendrimer-based nanopatterns on poly(Llactic acid) were used as bioactive substrates to evaluate the impact of the RGD local surface density on the chondrogenic induction of adult human mesenchymal stem cells. During chondrogenic commitment, active extracellular matrix (ECM) remodeling takes place, playing an instructive role in the differentiation process. Although three-dimensional environments such as pellet or micromass cultures are commonly used for in vitro chondrogenic differentiation, these cultures are rather limited with respect to their ability to interrogate cells in cell–ECM interactions. In the present study, the nanopatterns of the tunable RGD surface density were obtained as a function of the initial dendrimer concentration. The local RGD surface density was quantified through probability contour plots for the minimum interparticle distance, constructed from the corresponding atomic force microscopy images, and correlated with the cell adhesion and differentiation response. The results revealed that the local RGD surface density at the nanoscale acts as a regulator of chondrogenic commitment, and that intermediate adhesiveness of cells to the substrates favors mesenchymal cell condensation and early chondrogenic differentiation.
Tsinghua University Press
Anna Lagunas,Iro Tsintzou,Yolanda Vida,Daniel Collado,Ezequiel Pérez-Inestrosa,Cristina Rodríguez Pereira,Joana Magalhaes,José A. Andrades,Josep Samitier, Tailoring RGD local surface density at the nanoscale toward adult stem cell chondrogenic commitment. NanoRes.2017, 10(6): 1959–1971