A nano- and micro- integrated protein chip based on quantum dot probes and a microfluidic network
Quantum dots, microfl uidics, carcinoma embryonic antigen (CEA), microarray, polydimethylsiloxane (PDMS)
A novel nano- and micro-integrated protein chip (NMIPC) that can detect proteins with ultrahigh sensitivity has been fabricated. A microfl uidic network (μFN) was used to construct the protein chips, which allowed facile patterning of proteins and subsequent biomolecular recognition. Aqueous phase-synthesized, water-soluble fl uorescent CdTe/CdS core-shell quantum dots (aqQDs), having high quantum yield and high photostability, were used as the signaling probe. Importantly, it was found that aqQDs were compatible with microfluidic format assays, which afforded highly sensitive protein chips for cancer biomarker assays.
Tsinghua University Press
Juan Yan,Mei Hu,Di Li,Yao He,Rui Zhao,Xingyu Jiang,Shiping Song,Lianhui Wang,Chunhai Fan, A nano- and micro- integrated protein chip based on quantum dot probes and a microfluidic network. NanoRes.2008, 1: 490-496