Article Title

A nano- and micro- integrated protein chip based on quantum dot probes and a microfluidic network

Authors

Juan Yan, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
Mei Hu, Laboratory of Advanced Materials (LAM), Fudan University, 220 Handan Road, Shanghai 200433, China
Di Li, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
Yao He, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China Laboratory of Advanced Materials (LAM), Fudan University, 220 Handan Road, Shanghai 200433, China
Rui Zhao, National Center for NanoScience and Technology, No. 11, Beiyitiao, Zhongguancun, Beijing 100080, China
Xingyu Jiang, National Center for NanoScience and Technology, No. 11, Beiyitiao, Zhongguancun, Beijing 100080, China
Shiping Song, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
Lianhui Wang, Laboratory of Advanced Materials (LAM), Fudan University, 220 Handan Road, Shanghai 200433, China
Chunhai Fan, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China

Keywords

Quantum dots, microfl uidics, carcinoma embryonic antigen (CEA), microarray, polydimethylsiloxane (PDMS)

Abstract

A novel nano- and micro-integrated protein chip (NMIPC) that can detect proteins with ultrahigh sensitivity has been fabricated. A microfl uidic network (μFN) was used to construct the protein chips, which allowed facile patterning of proteins and subsequent biomolecular recognition. Aqueous phase-synthesized, water-soluble fl uorescent CdTe/CdS core-shell quantum dots (aqQDs), having high quantum yield and high photostability, were used as the signaling probe. Importantly, it was found that aqQDs were compatible with microfluidic format assays, which afforded highly sensitive protein chips for cancer biomarker assays.

Graphical Abstract

DOI

https://doi.org/10.1007/s12274-008-8052-1

Publisher

Tsinghua University Press

Recommended Citation

Juan Yan,Mei Hu,Di Li,Yao He,Rui Zhao,Xingyu Jiang,Shiping Song,Lianhui Wang,Chunhai Fan, A nano- and micro- integrated protein chip based on quantum dot probes and a microfluidic network. NanoRes.2008, 1: 490-496