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Brain Science Advances

Authors

Wei Chen, Biomanufacturing Center, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China; Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Beijing 100084, China; "Biomanufacturing and Engineering Living Systems" Innovation International Talents Base (111 Base), Beijing 100084, China
Ke Gai, Biomanufacturing Center, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China; Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Beijing 100084, China; "Biomanufacturing and Engineering Living Systems" Innovation International Talents Base (111 Base), Beijing 100084, China
Feng Lin, Biomanufacturing Center, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China; Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Beijing 100084, China; "Biomanufacturing and Engineering Living Systems" Innovation International Talents Base (111 Base), Beijing 100084, China
Wei Sun, Biomanufacturing Center, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China; Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Beijing 100084, China; "Biomanufacturing and Engineering Living Systems" Innovation International Talents Base (111 Base), Beijing 100084, China; Department of Mechanical Engineering, Drexel University, Philadelphia, PA, USA
Yu Song, Biomanufacturing Center, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China; Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Beijing 100084, China; "Biomanufacturing and Engineering Living Systems" Innovation International Talents Base (111 Base), Beijing 100084, China

Keywords

primary neural cells, neural stem cells, neural tissue-like constructs, biomaterials, 3D bioprinting

Abstract

Neural tissue-like constructs have important application potential in both neural tissue regeneration and individual medical treatment due to the ideal bioenvironment they provide for the growth of primary and stem cells. The biomaterials used in three-dimensional (3D) biomanufacturing techniques play a critical role in bioenvironment fabrication. They help optimize the manufacturing techniques and the long-term environment that supports cell structure and nutrient transmission. This paper reviews the current progress being made in the biomaterials utilized in neural cell cultures for in vitro bioenvironment construction. The following four requirements for biomaterials are evaluated: biocompatibility, porosity, supportability, and permeability. This study also summarizes the recent culture models based on primary neural cells. Furthermore, the biomaterials used for neural stem cell constructs are discussed. This study’s results indicate that compared with traditional two-dimensional (2D) cultures (with minimal biomaterial requirements), modulus 3D cultures greatly benefit from optimized biomaterials for long-term culturing.

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